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ProteomicsCo-IP of v-ATPase subcomplexes from MEFs under amino acid starvation and restimulation
v-ATPase Vo and V1 domains were isolated via Co-IP from MEFs expressing HA-Atp6V1B2 and FLAG-Atp6VoaA3 using HA- and FLAG-tag antibodies. Treatment groups were full medium (control), amino acid starvation (60 min) and restimulation (30 min). Cells were grown in SILAC medium for 3 passages.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-20 | PXD030174 | Pride
Mefs Co-ip Silac Vatpase Amino acid starvation 
ProteomicsLysosomal fractions from MEFs under amino acid starvation and restimulation
Magnetically enriched lysosomal fractions from MEFs were prepared from cells in three different conditions: full media (control), 1 h amino acid starvation and 30 min amino acid restimulation.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-20 | PXD030172 | Pride
Mefs Mouse Lysosomes Amino acid starvation 
ProteomicsLYSET/TMEM251 KO and GNPTAB KO secretomes from MEFs
Analysis of proteins secreted over 24 h in OptiMEM by LYSET/TMEM251 knockout, GNPTAB knockout and wild-type MEFs
ORGANISM(S): Mus musculus (Mouse) 
2022-09-29 | PXD035822 | Pride
Mefs Lyset Secretome Tmem251 Gnptab 
ProteomicsLYSET/TMEM251 SILAC Co-IP from MIA PaCA-2
Endogenous LYSET/TMEM251 was isolated via Co-IP from MIA PaCa-2 cells. LYSET KO cells were used as controls. Cells were grown in SILAC medium for 3 passages
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD035821 | Pride
Silac co-ip Human Lyset Tmem251 
ProteomicsLYSET/TMEM251 KO cellular and lysosomal proteomes from MIA PaCa-2 cells
Proteome analysis of post-nuclear supernatants (PNS) and enriched lysosomal fractions from LYSET/TMEM251 knockout and control MIA PaCa-2 cells. Lysosomes were loaded with iron nanoparticles (DexoMAG C, Liquids Research). PNS were generated from cell homogenates and used as input to enrich lysosomal...
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD029659 | Pride
Pns Lysosomes Human Pancreatic cancer 
ProteomicsLYSET/TMEM251 KO secretome from MIA PaCa-2 cells
Analysis of proteins secreted over 24 h in OptiMEM by LYSET/TMEM251 knockout and control MIA PaCa-2 cells.
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD029658 | Pride
Human Pancreatic cancer Secretome 
UnknownMultiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase.
Not available
S-EPMC9361285 | biostudies-literature
UnknownSARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study.
Not available
S-EPMC7995808 | biostudies-literature
UnknownTiming of surgery following SARS-CoV-2 infection: an international prospective cohort study.
Not available
S-EPMC8206995 | biostudies-literature
UnknownSARS-CoV-2 infection and venous thromboembolism after surgery: an international prospective cohort study.
Not available
S-EPMC8652887 | biostudies-literature
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