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TranscriptomicsParamecium tetraurelia autogamy series 4
In the ciliate Paramecium tetraurelia, autogamy is a self-fertilization process, during which the zygotic nucleus results from the fusion of two identical gametic nuclei. This phenomenon occurs in response to starvation. It starts with meiosis of the germline nuclei (micronuclei or MIC) and fragment...
ORGANISM(S): Paramecium tetraurelia 
2010-07-07 | E-GEOD-17997 | biostudies-arrayexpress
TranscriptomicsParamecium tetraurelia autogamy series 3
In the ciliate Paramecium tetraurelia, autogamy is a self-fertilization process, during which the zygotic nucleus results from the fusion of two identical gametic nuclei. This phenomenon occurs in response to starvation. It starts with meiosis of the germline nuclei (micronuclei or MIC) and fragment...
ORGANISM(S): Paramecium tetraurelia 
2010-07-07 | E-GEOD-17998 | biostudies-arrayexpress
TranscriptomicsParamecium tetraurelia autogamy series 1
In the ciliate Paramecium tetraurelia, autogamy is a self-fertilization process, during which the zygotic nucleus results from the fusion of two identical gametic nuclei. This phenomenon occurs in response to starvation. It starts with meiosis of the germline nuclei (micronuclei or MIC) and fragment...
ORGANISM(S): Paramecium tetraurelia 
2010-07-07 | E-GEOD-17996 | biostudies-arrayexpress
ProteomicsRNA-mediated nucleosome depletion is required for elimination of transposon-derived DNA
To study the role of chromatin remodeler during programmed genome reorganization in Paramecium tetraurelia. Paramecium ISWI1 was tagged with 3 FLAG and HA at its C-terminal. The recombinant plasmid was transformed into Paramecium and used for co-immunoprecipitation and Mass spectrometry studies to i...
ORGANISM(S): Paramecium tetraurelia 
2023-03-11 | PXD027206 | Pride
Paramecium tetraurelia Transposable elements Genome reorganization Nucleosome positioning Epigenetics project Iswi Chromatin 
GenomicsParamecium tetraurelia strain d4-2
The macronuclear genome of Paramecium tetraurelia has been sequenced by the Paramecium Genomics Consortium
PRJNA12726 | ENA
ProteomicsParamecium Gtsf1 is an essential nuclear PIWI-interacting protein controlling the selective degradation of small RNAs during programmed DNA elimination
Ciliates undergo developmentally programmed genome elimination, in which small RNAs direct the removal of target DNA segments, including transposable elements. At each sexual generation, the development of the macronucleus (MAC) requires massive and reproducible elimination of a large proportion of ...
ORGANISM(S): Paramecium triaurelia 
2024-10-17 | PXD045266 | Pride
Piwi Srna Gtsf1 Paramecium 
ProteomicsMass spectrometry of PtGtsf1 interacted proteins in Paramecium tetraurelia
PtGtsf1 was fused with FlagHA and induced to express in Paramecium tetraurelia. The interacted proteins of PtGtsf1 was obtained by immunoprecipitation with anti-HA antibody, followed with mass spectrometry to identify the proteins. Meanwhile, FlagHA without PtGtsf1 was expressed in Paramecium tetrau...
ORGANISM(S): Paramecium tetraurelia 
2025-05-06 | PXD049029 | Pride
Ptgtsf1 Paramecium tetraurelia Mass spectrometry 
ProteomicsThe Paramecium Ezl1 protein mediates dual methylation of H3 lysines 9 and 27
The goal of the present project was to determine which Paramecium histone H3 residues are methylated in an Ezl1-dependent manner.
ORGANISM(S): Paramecium tetraurelia 
2019-06-26 | PXD012170 | Pride
Histone Methylation Polycomb 
ProteomicsA histone methyltransferase-independent function of PRC2 controls small RNA dynamics during programmed DNA elimination in Paramecium
To limit transposable element (TE) mobilization, most eukaryotes have evolved small RNAs to silence TE activity via homology-dependent mechanisms. Small RNAs, 20-30 nucleotides in length, bind to PIWI proteins and guide them to nascent transcripts by sequence complementarity, triggering the recruitm...
ORGANISM(S): Paramecium tetraurelia 
2025-09-22 | PXD063336 | Pride
Histone methyltransferase Programmed dna elimination Prc2 Small rna Paramecium 
GenomicsParamecium tetraurelia
Paramecium tetraurelia Transcriptome or Gene expression
PRJNA269953 | ENA
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