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ProteomicsDistinct branches of the N-end rule pathway modulate the plant immune response
The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, though little information exists regarding biological roles. Here we show that the cysteine (Cys-) and glutamine (Gln)-specific amino-terminal (Nt)-amidase NTAQ1 branches are both components of the plant imm...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-11-06 | PXD008520 | Pride
Ntaq N-end rule pathway Plant immunity Erfvii Amino-terminal glutamine amidase 
ProteomicsDYn-2 based identification of Arabidopsis sulfenomes
Identifying the sulfenylation state (-SOH) of stressed cells. Here, we optimized an in vivo trapping method of sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells. With click chemistry, the dimedone based alkyne functionalized DYn-2 probe was biotinylated for subsequent streptavidin enri...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-06-27 | PXD001562 | Pride
Arabidopsis Sulfenome Dyn-2 click chemistry H2o2 Ros Biotinylation 
ProteomicsComparison of the MCF7 proteome cultured in standard FBS and EV depleted FBS
COmparison of the MCF7 proteomes after being cultured for 3 d in either 10% FBS (standard, commercial) or in 10% EV-depleted FBS (EDS, commercial) to validate the absence of EV specific proteins in EDS to exclude potential cross-contamination of xeno-EVs
ORGANISM(S): Bos taurus (Bovine) 
2025-05-06 | PXD051956 | Pride
Ev-depleted fbs Extracellular vesicles Starvation Fbs 
ProteomicsMCF7 proteome of cells and EVs during starvation
Serum deprivation during cell culture is common in EV research in oder to omit the introduction of xeno-EV material and reduce the presence of contaminating non-EV proteins from bovine serum. However, starvation has a profound effect on the cell proteome. Here, we provide proteome data that shows la...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD051955 | Pride
Mcf7 Starvation Extracellular vesicles 
ProteomicsFilter-aided extracellular vesicle enrichment (FAEVEr) for proteomics
Comaprison of the extracellular vesicle proteome using Filter-Aided Extracellular vescile Enrichment (FAEVEr) and ultracentrifugation. During enrichment different percentages of TWEEN-20 were supplemented in the wash buffer.
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-05-06 | PXD051938 | Pride
Ultracentrifugation Ev enrichment Optimization Ultrafiltration 
ProteomicsFAEVEr on biological fluids plasma and urine
Isolation of EVs from complex biofluids plasma and urine using the filter-aided EV enrichment strategy in combination with TWEEN-20 washes
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD056479 | Pride
Plasma Urine Extracellular vesicles 300 kda mwco 
ProteomicsProteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Despite the growing interest in using chemical genetics in plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution massspectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD030309 | Pride
Brassinosteroids Auxin Chemical genetics Cellular thermal shift assay 
ProteomicsLFQ comparison of total cell lysate, lysosomal and plasma membrane fraction of WT and NPC1 KO cells
Superparamagnetic iron oxide nanoparticles (SPIONs) have so far mainly been used as cellular carriers for genes and therapeutic products, while their use in subcellular organelle isolation remains largely underexploited. We engineered surface functionalized SPIONs (Ø 10 nm) that target very distinc...
ORGANISM(S): Homo sapiens (Human) 
2017-02-01 | PXD004273 | Pride
Lysosomal fraction Niemann-pick disease type c1 Label-free quantification Superparamegnetic iron oxide nanoparticles 
ProteomicsA preclinical platform for assessing long-term drug efficacy exploiting mechanically tunable scaffolds colonized by a three-dimensional tumor microenvironment
Background: Long-term drug evaluation heavily relies upon rodent models. Drug discovery methods to reduce animal models in oncology may include three-dimensional (3D) cellular systems that take into account tumor microenvironment (TME) cell types and biomechanical properties. Methods: In this study...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD039590 | Pride
3d cancer model  long-term  drug evaluation  pre-clinical  micro-environment  stiffness 
ProteomicsThe auophagy receptor SQSTM1/p62 mediates anti-inflammatory actions of the selective NR3C1/glucocorticoid receptor modulator compound A (CpdA) in macrophages
Glucocorticoids are widely used to treat inflammatory disorders; however, prolonged use of glucocorticoids results in side effects including osteoporosis, diabetes and obesity. Compound A (CpdA), identified as a selective NR3C1/glucocorticoid receptor (nuclear receptor subfamily 3, group C, member 1...
ORGANISM(S): Mus musculus (Mouse) 
2019-06-11 | PXD012121 | Pride
Autophagy  autophagy receptors  cpda  glucocorticoids  inflammation  nfe2l2/nrf2  sqstm1/p62 
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