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ProteomicsThe nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols
In aerobic life forms, reactive oxygen species (ROS) are produced by the partial reduction of oxygen during energy-generating metabolic processes. In plants, ROS production increases during periods of both abiotic and biotic stress, severely overloading the antioxidant systems. Hydrogen peroxide (H2...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-02-20 | PXD057987 | Pride
Sulfenylation Arabidopsis Gcn5 Dimedone 
ProteomicsPeroxisome-derived hydrogen peroxide can modulate the sulfenylation profiles of key redox signaling proteins in Flp-In T-REx 293 cells
Ever since the first characterization of peroxisomes, a central theme has been their involvement in cellular hydrogen peroxide (H2O2) metabolism. While the reputation of H2O2 drastically changed from an exclusively toxic molecule to a signaling messenger, the regulatory role of peroxisomes in these ...
ORGANISM(S): Homo sapiens (Human) 
2022-05-10 | PXD030782 | Pride
Peroxisome  hydrogen peroxide  cysteine thiol group  yap1c-based sulfenome mining  peroxiredoxin  mitochondrion 
UnknownReactivity, Selectivity, and Stability in Sulfenic Acid Detection: A Comparative Study of Nucleophilic and Electrophilic Probes.
Not available
S-EPMC4886738 | biostudies-literature
UnknownReaction-based fluorogenic probes for detecting protein cysteine oxidation in living cells.
Not available
S-EPMC9492777 | biostudies-literature
UnknownProfiling the Reactivity of Cyclic C-Nucleophiles towards Electrophilic Sulfur in Cysteine Sulfenic Acid.
Not available
S-EPMC4724439 | biostudies-literature
UnknownPeroxisome-Derived Hydrogen Peroxide Modulates the Sulfenylation Profiles of Key Redox Signaling Proteins in Flp-In T-REx 293 Cells.
Not available
S-EPMC9086853 | biostudies-literature
ProteomicsDYn-2 based identification of Arabidopsis sulfenomes
Identifying the sulfenylation state (-SOH) of stressed cells. Here, we optimized an in vivo trapping method of sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells. With click chemistry, the dimedone based alkyne functionalized DYn-2 probe was biotinylated for subsequent streptavidin enri...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-06-27 | PXD001562 | Pride
Arabidopsis Sulfenome Dyn-2 click chemistry H2o2 Ros Biotinylation 
ProteomicsReaction-based fluorogenic probes for selective detection of cysteinyl oxidation in living cells
Measuring reactive oxygen, nitrogen and sulfur species in cells is established technology, but turn-on fluorescence tools for detecting the products of their reaction with protein cysteines remain essentially unknown. Toward this goal, here we describe fluorogenic probes for sulfenic acid, a redox ...
ORGANISM(S): Homo Sapiens 
PXD029176 |
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