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ProteomicsCell rearrangement during angiogenesis requires suppression of actomyosin at cell-cell contacts via PI3-kinase.
Sprouting angiogenesis is a highly dynamic process which relies on the continuous interchange of endothelial cell relative position within the vascular sprout, a process mediated by cell rearrangements. Here we take advantage of a previous identified role of p110a/PI3K regulating endothelial cell mo...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-02 | PXD007060 | Pride
Mascot Pescal Phosphoproteomics Qexactive-plus Mice 
ProteomicsPhosphoproteomics data classify haematological cancer cell lines according to tumour type and sensitivity to kinase inhibitors
Acute myeloid leukemia, lymphoma and multiple myeloma cell lines were grown for 24h in RPMI medium and cells were harvested by centrifugation. Samples were digested with trypsin and subjected to phosphoenrichment using IMAC. Phosphopeptides were run in a LTQ-Orbitrap-XL. Peaks lists were generated ...
ORGANISM(S): Homo sapiens (Human) 
2013-04-26 | PXD000217 | Pride
Kinase inhibitor Drug sensitivity Imac Phosphoproteomics Hierarchical clustering analysis Blood malignancies Acute myeloid leukemia 
ProteomicsEnvironmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines
The breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition ...
ORGANISM(S): Homo sapiens (Human) 
2014-04-11 | PXD000472 | Pride
Label free Mcf7 Room temperature exposition Tio2 phosphoenrichment 
ProteomicsECM-enriched proteomics of human fallopian tube, fimbria and ovarian tissue
We analysed the extracellular matrix (ECM) landscape of fresh, healthy tissues from human fallopian tube (FT), fimbria (FB, the tissue of origin of serous tubal intraepithelial lesions) and ovarian tissue (OV). The aim was to identify differentially expressed matrix proteins between FB and FT or OV ...
ORGANISM(S): Homo sapiens (Human) 
2022-06-20 | PXD023912 | Pride
Ecm Ovarian cancer Tgfbi Fallopian tube Fimbria Ovary 
ProteomicsKinase Substrate Enrichment Analysis Provides Insights into the Heterogeneity of Signaling Pathway Activation in Leukemia Cells
Experiments were performed in AML (Acute Myeloid Leukemia) cell lines and in primary cells coming from patients with AML and healthy donors. The AML cell lines P31/Fuj and Kasumi-1 were treated with vehicle (DMSO), 100 nM or 1000 nM of the PI3K/mTOR inhibitor AZ123 or the mTOR inhibitor Ku0063794 f...
ORGANISM(S): Homo sapiens (Human) 
2013-04-09 | PXD000185 | Pride
Tio2 phosphoenrichment Label free quantification Aml primary cells Pi3k/mtor inhibitors 
ProteomicsResponse Marker Enrichment Analysis informs the impact of pharmacological and genetic perturbagens on cancer cells
The project focuses on the development and validation of our novel enrichment technique, Response Marker Enrichment Analysis (ReMEA). As part of this validation, we present two datasets consisting of MS/MS analysis of drug treated acute myeloid leukaemia (AML) cell lines. These are as follows: First...
ORGANISM(S): Homo sapiens (Human) 
2026-08-13 | PXD063643 | Pride
Aml Enrichment analysis Cancer Leukemia Drug treatment proteomics Phosphoproteomics Lc-ms/ms 
ProteomicsCharacterising early phosphorylation events upon HER2 protein overexpression
HER2 transduced cells which we will refer to as HER2-DOX –. These cells are 99% GFP positive (i.e., 99% cells have HER2 transduced, un-induced). As a control, we had GFP empty vector transduced MCF10A cells (95% have GFP transduced). Both cell types in triplicates. We had 4 time-points 0h, 30 mins, ...
ORGANISM(S): Homo sapiens (Human) 
2023-01-23 | PXD034105 | Pride
Dox Her2 Labell-fee Phosphopoproteomics Gfp Mcf10a Lc-ms/ms 
ProteomicsAdipocytes disrupt the translational programme of Acute Lymphoblastic Leukaemia to favour tumour survival and persistence
Nalm-6 cells co-cultured with 3T3-L1 adipocytes or pre-adipocytes were subjected to quantitative LC-MS/MS phosphoproteomic analysis at two timepoints (+24h and +72h) to determine dynamics of cell signalling. Two independent biological replicates were analysed per condition.
ORGANISM(S): Homo sapiens (Human) 
2021-06-09 | PXD019186 | Pride
Stress resistance Acute lymphoblastic leukaemia (all) Translation Adipocyte niche 
ProteomicsBRAF mutations disrupt the hypothalamo-pituitary axis leading to hypopituitarism in mouse and humans
Germline mutations in BRAF and other components of the Mitogen-Activated Protein Kinase (MAPK) pathway are associated with the congenital syndromes collectively known as RASopathies. Here, we report the association of Septo-Optic Dysplasia (SOD) including hypopituitarism and Cardio-Facio-Cutaneous (...
ORGANISM(S): Homo sapiens (Human) 
2020-11-17 | PXD018190 | Pride
Label free Ksea Phosphoproteomics Human Rasopathies Braf Lcms 
ProteomicsKinase activity ranking (KAR) quantifies the contribution of protein kinases to the regulation of cell viability
New method to determine kinase activity based on phosphoproteomics data
ORGANISM(S): Homo sapiens (Human) 
2017-07-06 | PXD005073 | Pride
Aml kinase phosphorylation 
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