Genes are pleiotropic and getting a better knowledge of their function requires a comprehensive characterization of their mutants. Here, we generated multi-level data combining phenomic, proteomic and metabolomic acquisitions from plasma and liver tissues of two C57BL/6 N mouse models lacking the...
We optimized a procedure of phosphotyrosine enrichment with immunoprecipitation. Then the optimized procedure was applied into a global analysis of phosphotyrosine status in culture cells treated with Erlotinib.
Protein tyrosine phosphatases (PTPs) cooperate with protein tyrosine kinases to regulate intracellular tyrosine phosphorylation levels, and their dysfunction is associated with various diseases. However, due to the low abundance of PTPs in cells, comprehensive analysis requires an enrichment step pr...
Phosphotyrosine (pY) enrichment is critical for expanding fundamental and clinical understanding of cellular signaling by mass spectrometry-based proteomics. However, current pY enrichment methods exhibit a high cost per sample and limited reproducibility due to expensive affinity reagents and manua...
KRAS is one of the most frequently mutated genes across all cancer subtypes. Two of the most frequent oncogenic KRAS mutations observed in patients result in glycine to aspartic acid substitution at either codon 12 (G12D) or 13 (G13D). Although the biochemical differences between these two predomina...
Phosphotyrosine status were compared between colorectal cancer cells which is sensitive or resistant to Cetuximab. Activated kinases in resistant cells were examined as targets to overcome the resistance.
Signal propagation mediating numerous cellular processed in T cells is orchestrated by tyrosine phosphorylation with a high degree of regulation1, 2. Because aberrant tyrosine phosphorylation can result in many diseases3, a method to systematically quantify this dynamic process can be helpful in und...
