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ProteomicsA Photocrosslinking Probe to Capture the Substrates of Caseinolytic Protease P
Protein homeostasis in bacteria is regulated by proteases such as the tetradecameric caseinolytic protease P (ClpP). Although substrates of ClpP have been successfully deciphered in genetically engineered cells, methods which directly trap processed proteins within native cells remain elusive. Here,...
ORGANISM(S): Staphylococcus aureus 
2024-10-04 | PXD047274 | Pride
S. aureus Substrates 
ProteomicsSseF-SscB Interaction - Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems
Bacterial type III secretion systems are able to secrete membrane proteins. In this project we could show that cognate T3SS chaperones are able to bind to the transmembrane segments of T3SS substrates to avoid erroneous targeting to the bacterial inner membrane. Here, we studied the Salmonella SPI-2...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2018-10-16 | PXD010470 | Pride
Salmonella Pbpa Sscb In vivo photocrosslinking Ssef Type iii secretion systems 
ProteomicsDevelopment of Photocrosslinking Probes Based on Huwentoxin-IV to Map the Site of Interaction on Nav1.7
This study reports on the design of a library of photoprobes based on the potent spider toxin Huwentoxin-IV and the determination of the toxin binding interface on VSD2 of Nav1.7 through a photocrosslinking and tandem mass spectrometry approach. Our Huwentoxin-IV probes selectively crosslink to extr...
ORGANISM(S): Homo sapiens (Human) 
2019-11-18 | PXD015037 | Pride
Nav1.7 Photoaffinity labelling Huwentoxin-iv Diazirine Peptide mapping 
ProteomicsMS analysis of photocrosslink adducts for the mapping of interaction sites of selective engineered PSD-95 PDZ domain ligands
Designing highly specific modulators of protein-protein interactions (PPIs) is especially challenging in the context of multiple paralogs and conserved interaction surfaces. In this case, direct generation of selective and competitive inhibitors is hindered by high similarity within the evolutionary...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-11-12 | PXD015366 | Pride
Engineered ligand/pdz domains/photocrosslinking/p-azidophenylalanine 
ProteomicsSubstrate profiling of mitochondrial caseinolytic protease P via a site-specific photocrosslinking approach
The function of proteases is mainly determined by the substrates they are processing. However, substrate identification remains a challenging task. Here, we used genetic code expansion to incorporate a diazirine-lysine amino acid (DiazK) at selected positions of mitochondrial caseinolytic protease P...
ORGANISM(S): Homo sapiens (Human) 
2023-08-22 | PXD027954 | Pride
Mtupr Mitochondria Photoaffinity crosslinking Clpxp Orbitrap fusion 
UnknownGenetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label.
Not available
S-EPMC4974458 | biostudies-literature
ProteomicsIdentification of proteins crosslinked to lysosomal sphingosine and cholesterol probes
HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid compl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-07 | PXD039780 | Pride
Lysosomal lipids Photoaffinity labelling Click chemistry 
ProteomicsPhotocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions
Almost all cellular processes are influenced by ubiquitination. A large family of enzymes known as E3 ligases provide the specificity for ubiquitination, with the largest class among them, the RING E3s, comprising over 600 members in humans. RING E3s facilitate transfer of ubiquitin to substrates by...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075613 | Pride
Ubiquitin n-maleimido-diazirine e3 ligase activity-based probe 
ProteomicsSpatial-temporal protein interactome profiling via condensation enhanced crosslinking
MS data for the verification of metabolic incorporation of δ-photolysine, calculation for the crosslinking distance by DegP crosslinking, and the proteomics analysis for the dynamic direct interactome of NPM1 as well as the SG-specific interactomes of SARS-CoV-2 Nucleocapsid protein and YTHDF1/2.
ORGANISM(S): Homo Sapiens Escherichia Coli Severe Acute Respiratory Syndrome Coronavirus 2 
PXD052099 |
UnknownDevelopment of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering.
Not available
S-EPMC10634525 | biostudies-literature
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