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ProteomicsA Spatial Interactome Reveals the Protein Organization of the Algal CO2 Concentrating Mechanism
One-third of global CO2 fixation is mediated by eukaryotic algae. Nearly all algae enhance their carbon assimilation by operating a CO2 concentrating mechanism (CCM), built around a mysterious organelle called the pyrenoid. Here, we leveraged new tools to determine the localizations of 146 candidate...
ORGANISM(S): Chlamydomonas reinhardtii 
2018-01-24 | PXD007664 | Pride
Co2-concentrating mechanism Ccm Carbon-fixation Photosynthesis Pyrenoid 15n isotopic labelling Rubisco High-throughput fluorescence protein tagging Orbitrap Affinity purification mass spectrometry Chlamydomonas reinhardtii Interactome 
UnknownThe versatile interactome of chloroplast ribosomes revealed by affinity purification mass spectrometry.
Not available
S-EPMC7797057 | biostudies-literature
UnknownA Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism.
Not available
S-EPMC5616186 | biostudies-literature
ProteomicsChemical crosslinking of photosynthetic protein complexes in Cyanidioschyzon merolae
Efficient photosynthetic electron transfer relies on transient interactions between photosystem I (PSI) and soluble electron carriers. However, structural information describing these transient interactions are limited in red algae. Here, we applied chemical crosslinking mass spectrometry (XL-MS) to...
ORGANISM(S): Cyanidioschyzon merolae 
2026-08-06 | PXD079525 | Pride
Cyanidioschyzon merolae Photosystem i Fnr Dss Crosslinking 
ProteomicsMapping Architecture of Protein complexes in Arabidopsis using XL-MS (Histone H2B turboID)
Capturing molecular machines in action is key to understanding protein complex architecture, cellular regulation, and gene function. Here, we present a cross-linking mass spectrometry (XL-MS) platform using the PhoX cross-linker to map proteome-wide interactions in Arabidopsis thaliana. By analyzing...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-07-29 | PXD078284 | Pride
Cross-linking mass spectrometry (xl-ms) Interlink Arabidopsis. Ribosome interactome Histone interactome Protein-protein interaction (ppi) network Cross-links Intralink Photosystem interactome 
ProteomicsFast and global reorganization of the chloroplast protein biogenesis network during heat acclimation
Heat waves caused by global warming severely challenge photosynthesis and hence plant biomass production, but the mechanisms of its adjustment, repair and biogenesis during heat acclimation are elusive. We provide a global and time-resolved insight, how these processes are modulated during heat accl...
ORGANISM(S): Chlamydomonas reinhardtii 
2022-01-06 | PXD024949 | Pride
Photosynthesis biogenesis Protein complex assembly Translational regulation Protein synthesis Heat acclimation Chloroplast 
UnknownInteraction network of tobacco etch potyvirus NIa protein with the host proteome during infection.
Not available
S-EPMC4735970 | biostudies-literature
ProteomicsThe proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network
In plant cells, chloroplast gene expression is predominantly controlled through regulation of translation. Fine-tuning of protein synthesis is vital for plant development, acclimation to environmental challenges and for the assembly of major protein complexes such as the photosynthesis machinery. Ho...
ORGANISM(S): Chlamydomonas reinhardtii 
2021-01-04 | PXD020377 | Pride
Ribosome Chlamydomonas rheinhardtii Lcms Chloroplast 
ProteomicsThe proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network - Effect of Crosslinking
In plant cells, chloroplast gene expression is predominantly controlled through regulationof translation. Fine-tuning of protein synthesis is vital for plant development, acclimation to environmental challenges and for the assembly of major protein complexes such as the photosynthesis machinery. How...
ORGANISM(S): Chlamydomonas reinhardtii 
2020-11-23 | PXD022210 | Pride
Chlamydomonas Plastide ribosome Ae-ms Lc-ms/ms 
TranscriptomicsRNA-Seq of control and RNPS1 knockdown with or without RNPS1 wildtype or RNPS1-RRM domain rescue in Flp-In T-REx 293 cells
RNPS1 is a splicing regulatory protein and a component of the ASAP/PSAP complex, which is associated with the exon junction complex and modulates alternative splicing. It was previously postulated that the isolated RRM domain of RNPS1 in complex with ASAP/PSAP is able to regulate certain alternative...
ORGANISM(S): Homo sapiens 
2022-05-06 | E-MTAB-10770 | biostudies-arrayexpress
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