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MetabolomicsPKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis
Metabolic reprogramming is a hallmark of cancer. Herein we discover that the key glycolytic enzyme pyruvate kinase M2 isoform (PKM2), but not the related isoform PKM1, is methylated by co-activator-associated arginine methyltransferase 1 (CARM1). PKM2 methylation reversibly shifts the balance of met...
2021-07-30 | MTBLS533 | MetaboLights
MetabolomicsThe effects of PKM2 modulation and hypoxia on the metabolic landscape of Alzheimer patient-derived induced neurons
Not available
ORGANISM(S): Homo sapiens 
ST002214 | MetabolomicsWorkbench
TranscriptomicsExpression data from PKM1 or PKM2 expressing mouse embryonic fibroblasts.
We profiled global gene expression for two separate lines of mouse embryonic fibroblasts and find that deletion of PKM2 and expression of PKM1 does not alter global gene expression profiles. We used microarrays to futher characterize the effects of PKM1 expression compared to PKM2 expression on glob...
ORGANISM(S): Mus musculus 
2015-05-01 | E-GEOD-60499 | biostudies-arrayexpress
TranscriptomicsGermline loss of PKM2 promotes metabolic distress and hepatocellular carcinoma
Alternative splicing of the Pkm gene product generates the PKM1 and PKM2 isoforms of pyruvate kinase, and PKM2 expression is closely linked to embryogenesis, tissue regeneration, and cancer. To interrogate the functional requirement for PKM2 during development and tissue homeostasis, we generated ge...
ORGANISM(S): Mus musculus 
2016-03-25 | E-GEOD-79535 | biostudies-arrayexpress
TranscriptomicsExpression profiling of E14 cells overexpressing Hk2/Pkm2
Purpose: In this study, we compared transcriptome profiling (RNA-seq) between normal mouse embryonic stem cell (E14) and Hexokinase2 (Hk2)/ Pyruvate Kinase M2 (Pkm2) overexpressed E14 cell. Result: Using an optimized data analysis workflow, we mapped over 4 billion sequence reads per sample to the m...
ORGANISM(S): Mus musculus 
2016-07-03 | E-GEOD-65246 | biostudies-arrayexpress
TranscriptomicsSmall molecule activation of PKM in cancer cells induces serine auxotrophy
Proliferating tumor cells use aerobic glycolysis to support their high metabolic demands. Paradoxically, increased glycolysis is often accompanied by expression of the lower activity PKM isoform, effectively constraining lower glycolysis. Here, we report the discovery of novel PKM activators with ...
ORGANISM(S): Homo sapiens 
2012-12-20 | E-GEOD-40191 | biostudies-arrayexpress
TranscriptomicsRegulation of Pkm2 by miR-122.
The study identified Pkm2 to be highly expressed in human embryonic stem cells and hepatocellular carcinoma cells, and targeted by a microRNA that is highly expressed in human primary hepatocytes. Gene expression patterns were compared from total RNA obtained from human embryonic stem cells, human p...
ORGANISM(S): Homo sapiens 
2014-06-08 | E-GEOD-29907 | biostudies-arrayexpress
ProteomicsIP-MS detected the interation protein with PKM2
Vascular endothelial cells (ECs) senescence correlates with the increase of cardiovascular diseases in ageing population. Although ECs rely glycolysis for energy production, little is known about the role of glycolysis in ECs senescence. Here, we report a critical role for glycolysis-derived serine ...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD035585 | Pride
Co-immunoprecipitation Pkm2 
ProteomicsFunctional cross-talk between allosteric effects of activating and inhibiting ligands underlies PKM2 regulation.
Allosteric regulation is central to the role of the glycolytic enzyme pyruvate kinase M2 (PKM2) in cancer metabolism. Multiple activating and inhibitory allosteric ligands regulate PKM2 activity by controlling the equilibrium between high activity tetramers and low activity dimers and monomers. Howe...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD010334 | Pride
Srm Prm Pkm2 Quantitation Qexactive 
ProteomicsHydrogen Sulfide Coordinates Glucose Metabolism Switch through Destabilizing Tetrameric Pyruvate Kinase M2
Cancer cells reprogram their glucose metabolic pathway from oxidative phosphorylation toward aerobic glycolysis. Pyruvate kinase M2 (PKM2), which converts phosphoenolpyruvate (PEP) to pyruvate, is considered the rate-limiting enzyme involved in cancer glucose metabolism. By reducing PKM2 enzyme acti...
ORGANISM(S): Homo sapiens (Human) 
2024-07-09 | PXD044925 | Pride
Glucose metabolism Pkm2 Lc-ms Oligomerization Sulfhydration Hydrogen sulfide 
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