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MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
ProteomicsOverexpression of the plastidial pseudo-protease AtFtsHi3 enhances drought tolerance while sustaining plant growth
With climate change, droughts are expected to be more frequent and severe, severely impacting plant biomass and quality. Here, we show that overexpressing the Arabidopsis gene AtFtsHi3 (FtsHi3OE) enhances drought-tolerant phenotypes without compromising plant growth. AtFtsHi3 encodes a chloroplast e...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-06-14 | PXD037172 | Pride
Lc-ms/ms based proteomics Pseudo-protease ftshi3 Drought tolerance Mathematical models of protein complex formation 
ProteomicsFunction of clpF in the clp protease complex
The plant plastid Clp machinery comprises a hetero-oligomeric ClpPRT proteolytic core, ATP-dependent ClpC,D chaperones and an adaptor protein, ClpS1. ClpS1 directs a subset of substrates to the Clp protease-chaperone complex for degradation, but substrate recognition and delivery mechanisms are unkn...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-04-09 | PXD002186 | Pride
Lc-msms Plant cell Arabidopsis thaliana Coip Clp complex Chloroplast Stroma 
ProteomicsAnalysis of isolated Plastoglobules from Arabidopsis thaliana WT and M48 null mutants.
We studied the function of PG localized protease, PGM48 both in vitro and in vivo during natural senescence in Arabidopsis thaliana.Our results show that PGM48 is a metalloprotease and a senescence inducible protein. In vitro assay by recombinant protein revealed a proteolytic activity. Quantitative...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-04-09 | PXD003684 | Pride
Lc-msms Plastoglobules Arabidopsis thaliana Ltq-orbitrap Protease Chloroplast Quantitative proteomics 
ProteomicsTime-resolved inducible knock-down of Clp protease subunits in tobacco identifies putative protease substrates
The Clp protease complex in plants is the one with the highest complexity through all life kingdoms. It is composed of 14 nuclear- and plastid-encoded subunits which are allocated in different rings across the complex. The proteolytic ring (P-ring; ClpP3-ClpP6) and non-proteolytic ring (R-ring; ClpR...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-22 | PXD008305 | Pride
Clp protease  chloroplast  protein degradation  protease substrate  nicotiana tabacum  inducible rnai  lc-msms  shotgun proteomics 
ProteomicsThe transcriptome, extracellular proteome and active secretome of agroinfiltrated N. benthamiana uncover an expanded protease repertoire
Background: The infiltration of disarmed Agrobacterium tumefaciens into leaves of Nicotiana benthamiana (agroinfiltration) is a widely-adopted procedure to produce and study proteins in plant science, for instance to determine subcellular localization and protein-protein interactions. Agroinfiltrati...
ORGANISM(S): Agrobacterium fabrum str. C58 Nicotiana benthamiana 
2017-10-24 | PXD006708 | Pride
Activity-based proteomics Rnaseq Agroinfiltration Extracellular proteome Post-translational activation Silencing inhibitor p19 Plant immunity Nicotiana benthamiana Activity-based protein profiling (abpp) Agrobacterium tumefaciens Plant protease family Plant protease annotation Chlorosis 
ProteomicsSeparation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes
Conventional preparation methods of plant ribosomes fail to resolve non-translating chloroplast or cytoplasmic ribosome subunits from translating fractions. We established preparation of these ribosome complexes from Arabidopsis thaliana leaf, root, and seed tissues by optimized sucrose density grad...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-09-09 | PXD019329 | Pride
Plant proteomics Tissue specific separation Ribosome complexes Ribo-proteome In vivo protein complex stabilization 
ProteomicsGrafting systems for plant cadmium research: insights for basic plant physiology and applied mitigation
Cadmium (Cd) is a highly toxic and carcinogenic pollutant that poses a threat to human and animal health by affecting several major organ systems. Urbanization and human activities have led to significant increases in Cd concentration in the environment, including in agroecosystems. To protect again...
ORGANISM(S): Solanum lycopersicum 
2023-06-26 | PXD041184 | Pride
Abiotic stress  cadmium mitigation  environmental research  heavy metals  plant grafting  stress tolerance 
ProteomicsOverexpression of McHB7 Transcription Factor from Mesembryanthemum crystallinum Improves Plant Salt Tolerance
Mesembryanthemum crystallinum (common ice plant) is one of the facultative halophyte plants, and it serves as a model for investigating the molecular mechanisms underlying its salt stress response and tolerance. Here we cloned one of homeobox transcription factor (TF) gene McHB7 from ice plant, whic...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-08-11 | PXD033886 | Pride
Salt stress common ice plant proteomics 
ProteomicsA new cost-effective ribosome profiling technique complements the functional genome annotation and reveals a key role of genic 3’ untranslated region in plant translatomic variation
A new cost-effective ribosome profiling technique complements the functional genome annotation and reveals a key role of genic 3’ untranslated region in plant translatomic variation
ORGANISM(S): Zea mays (Maize) 
2021-05-30 | PXD019635 | Pride
Eqtl Maize 3'ribo-seq Protein ms 
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