Sort   by:  
 Page size 
ProteomicsDe Novo Sequencing of Polyclonal Antibodies by Integrating Intact Mass, Top-Down and Bottom-Up Mass Spectrometry
Antibodies are specialized proteins produced by the adaptive immune system to identify and neutralize harmful antigens. Antibody-based diagnostics and therapeutics have advanced rapidly, with recombinant antibodies derived from monoclonal antibodies (mAbs) becoming the preferred standard due to thei...
ORGANISM(S): Homo sapiens (Human) 
2025-10-31 | PXD063526 | Pride
Intact mass De novo sequencing Top-down Bottom-up Polyclonal antibody sequencing 
ProteomicsMurine IgG (polyclonal) from Listeria Challenged pregnant dams
IgG was collected from virgin and pregnant listeria challenged mouse plasma. Polyclonal IgG was analyzed by reduction, alkylation, and LC-MS with top-down MS/MS (HCD and ETD)to look for glycosylation PTMs
ORGANISM(S): Mus musculus (Mouse) 
2022-06-28 | PXD033357 | Pride
Polyclonal Igg Listeria Murine Igg2 
ProteomicsDeep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite de novo Protein Sequencing by Mass Spectrometry
Label-free protein sequencing is critically enabled by bottom-up, mass spectrometry-based proteomics workflows. Applications such as antibody sequencing or antigen discovery require de novo reconstruction of peptide and protein sequences. While trypsin has long served as the gold-standard protease i...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD063988 | Pride
Hta-proteases Hybrid fragmentation by eacid De novo antibody sequencing Bottom-up proteomics 
TranscriptomicsUSR_template_replicates
Next-generation sequencing (NGS) of antibody variable regions has emerged as a powerful tool in systems immunology by providing quantitative molecular information on polyclonal humoral immune responses. Reproducible and robust information on antibody repertoires is valuable for basic and applied imm...
ORGANISM(S): Mus musculus 
2013-10-26 | E-MTAB-1896 | biostudies-arrayexpress
ProteomicsBenchmarking of mass spectrometry-based antibody repertoire profiling reveals extensive systematic biases
The serum antibody repertoire is crucial for long-term immune protection, holding significant immunological and biotechnological value. Bottom-up proteomics is the most widely used mass spectrometry (MS) technique for profiling the sequence diversity of serum antibodies. However, serum antibody bott...
ORGANISM(S): Homo sapiens (Human) 
2025-12-04 | PXD055846 | Pride
Serum antibody bottom-up proteomics (ab-seq) Ms Antibody sequencing Serum antibody repertoire 
UnknownSimultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry.
Not available
S-EPMC12017766 | biostudies-literature
UnknownMapping polyclonal antibody responses to bacterial infection using next generation phage display.
Not available
S-EPMC4829855 | biostudies-literature
Unknown[Prokaryotic expression and purification of human TLE1 N-terminal Q domain fragment and production of its polyclonal antibody].
Not available
S-EPMC6000491 | biostudies-literature
ProteomicsIdentification of broadly inhibitory anti-PfEMP1 antibodies by mass spectrometry sequencing of plasma IgG from a malaria-exposed child
Plasmodium falciparum pathology is driven by the accumulation of parasite-infected erythrocytes in blood capillaries. We demonstrate a novel approach to identify pathogen-specific human monoclonal antibodies directly from plasma, combining mass-spectrometry analysis of antigen-purified polyclonal pl...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD064525 | Pride
Human Lc-msms Igg Plasma Malaria Antibody 
GenomicsParallel genomic alterations of antigen and payload targets mediate polyclonal acquired clinical resistance to the antibody drug conjugate sacituzumab govitecan
Parallel genomic alterations of antigen and payload targets mediate polyclonal acquired clinical resistance to the antibody drug conjugate sacituzumab govitecan
PRJNA754210 | ENA
Sort   by:  
 Page size