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ProteomicsThe dimeric deubiquitinase Usp28 integrates 53bp1 and Myc functions to limit DNA damage
The oncogenic transcription factor Myc is a pleiotropic regulator of RNA Polymerase II (RNAPII)-dependent transcription, DNA replication and DNA damage response pathways. Myc is stringently regulated by the ubiquitin system - for example, ubiquitination controls recruitment of the elongation factor ...
ORGANISM(S): Homo sapiens (Human) 
2023-11-09 | PXD037263 | Pride
Human Ap-ms Immunoprecipitation Usp28 53bp1 
ProteomicsHUWE1 interaction partners in HCT116 cells (TMT)
Deregulation of RNA Polymerase II (RNAPII) by oncogenic signaling leads to collisions of RNAPII with DNA synthesis machinery (transcription-replication conflicts, TRCs). TRCs can result in DNA damage and underlie genomic instability in tumor cells. Here we provide evidence that elongating RNAPII pro...
ORGANISM(S): Homo sapiens (Human) 
2023-07-31 | PXD037677 | Pride
Human Wrnip1 Hct116 Huwe1 
ProteomicsHUWE1 interaction partners in HCT116 cells (label free)
Deregulation of RNA Polymerase II (RNAPII) by oncogenic signaling leads to collisions of RNAPII with DNA synthesis machinery (transcription-replication conflicts, TRCs). TRCs can result in DNA damage and underlie genomic instability in tumor cells. Here we provide evidence that elongating RNAPII pro...
ORGANISM(S): Homo sapiens (Human) 
2023-07-31 | PXD037812 | Pride
Human Wrnip1 Hct116 Huwe1 
ProteomicspSer2-RNAPII interaction partners in MEF cells (label free)
Deregulation of RNA Polymerase II (RNAPII) by oncogenic signaling leads to collisions of RNAPII with DNA synthesis machinery (transcription-replication conflicts, TRCs). TRCs can result in DNA damage and underlie genomic instability in tumor cells. Here we provide evidence that elongating RNAPII pro...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-31 | PXD037680 | Pride
Ap-ms Mef Mouse Pser2-rnapii 
TranscriptomicsTranscription profiling of human carcinoma LS174T cells following depletion of Myc and Usp28 using shRNA
Analysis of gene expression following depletion of Myc and Usp28 using shRNA in colon carcinoma LS174T cells.
ORGANISM(S): Homo sapiens 
2007-04-19 | E-MEXP-1070 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of HectH9 in human osteosarcoma samples to identify myc regulated genes
Analysis of Gene Regulation by Myc in the absence of HectH9
ORGANISM(S): Homo sapiens 
2005-10-17 | E-MEXP-406 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of AURKA in human neuroblastoma IMR-32 cells
IMR-32 cells were infected either with control-shRNA or one of two different AURKA-shRNA vectors to identify genes regulated after AURKA knockdown.
ORGANISM(S): Homo sapiens 
2008-06-01 | E-MEXP-1496 | biostudies-arrayexpress
TranscriptomicsDual regulation of Fbw7 function and oncogenic transformation by Usp28
Fbw7, the substrate recognition subunit of SCF(Fbw7) ubiquitin ligase, mediates turnover of multiple proto-oncoproteins and promotes its own degradation. Fbw7-mediated substrate degradation is antagonized by the Usp28 deubiquitinase. We now show, using knockout mice, that Usp28 preferentially deubiq...
ORGANISM(S): Mus musculus 
2015-05-01 | E-GEOD-59354 | biostudies-arrayexpress
TranscriptomicsTumor cell-specific inhibition of MYC function using small molecule inhibitors of the HUWE1 ubiquitin ligase
Deregulated expression of MYC is a driver of colorectal carcinogenesis, necessitating novel strategies to inhibit MYC function. The ubiquitin ligase HUWE1 (HECTH9, ARFBP1, MULE) associates with both MYC and the MYC-associated protein MIZ1. We show here that HUWE1 is required for growth of colorecta...
ORGANISM(S): Homo sapiens 
2014-10-21 | E-GEOD-59223 | biostudies-arrayexpress
TranscriptomicsUbiquitin-dependent turnover of MYC promotes loading of the PAF complex on RNA Polymerase II to drive transcriptional elongation (RNA-seq)
The MYC transcription factor is an unstable protein and its turnover is controlled by the ubiquitin system. Ubiquitination enhances MYC-dependent transactivation, but the underlying mechanism remains unresolved. Here we show that proteasomal turnover of MYC is dispensable for recruitment of RNA poly...
ORGANISM(S): Homo sapiens 
2016-01-04 | E-GEOD-70000 | biostudies-arrayexpress
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