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ProteomicsCross-Linking Mass Spectrometry (CXMS) of human pre-60S ribosomal particles
Cross-Linking Mass Spectrometry (CXMS) analysis of human pre-60S ribosomal particles.
ORGANISM(S): Homo Sapiens 
PXD019025 |
ProteomicsRbp95 binds 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation
Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles requires structural remodeling by the Npa1 complex, but i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD030106 | Pride
Ribosome assembly factor Yeast S. cerevisiae Tandem-affinity purification (tap) Npa1 Pre-60s particles Rbp95 
ProteomicsEffect of Nop53 depletion on the compositional changes during pre-60S maturation
The eukaryotic ribosome biogenesis is a highly orchestrated multistep process that starts at the nucleolus with the transcription of pre-rRNAs 5S and 35S. The latter comprises the mature 18S, 5.8S and 25S rRNAs separated by internal transcribed spacers (ITS1 and ITS2) and externally flanked by the 5...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD023383 | Pride
Nop53 Eukaryotic ribosome biogenesis Pre-60s S. cerevisiae 
ProteomicsDiverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomal particles
Ribosome biogenesis is a highly complex process in eukaryotes, involving temporally and spatially regulated ribosomal protein (r-protein) binding and rRNA remodeling events in the nucleolus, nucleoplasm and cytoplasm. Hundreds of assembly factors (AFs), organized into sequential functional groups, f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-06-01 | PXD003736 | Pride
Assembly factors Cryo-em Pre-60s ribosome Cxms 
ProteomicsStructure of the AAA-ATPase Drg1 during extraction of Rlp24 from pre-60S particles
The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The mol...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-14 | PXD032098 | Pride
Large ribosomal subunit Ribosome biogenesis Aaa-atpases Substrate recognition Substrate translocation mechanism Single particle cryo-em 
UnknownVisualizing the nucleoplasmic maturation of human pre-60S ribosomal particles.
Not available
S-EPMC10624882 | biostudies-literature
ProteomicsIdentification of 60S pre-rRNA binding sites of human GNL3L GTPase
We reveal that the GNL3 paralogue GTPase, GNL3L, associates with pre-ribosomes. We map RNA contact sites on GNL3L as well as GNL3L binding sites on pre-rRNAs. Lack of GNL3L impairs synthesis of the 60S rRNAs and expression of GTPase inactive GNL3L causes defects in early steps of pre-rRNA processing...
ORGANISM(S): Homo sapiens (Human) 
2026-03-05 | PXD067606 | Pride
Human Gtpase Gnl3l 60s rrna Rna-protein crosslinking 
UnknownStructural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1.
Not available
S-EPMC5555584 | biostudies-literature
UnknownMak5 and Ebp2 act together on early pre-60S particles and their reduced functionality bypasses the requirement for the essential pre-60S factor Nsa1.
Not available
S-EPMC3846774 | biostudies-literature
ProteomicsDistinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases, including GNL3L
Production of the eukaryotic ribosomal subunits (40S and 60S) is a highly dynamic process in which numerous assembly factors coordinate structural rearrangements of pre-ribosomal complexes to achieve their mature, functional architectures. Across the domains of life, GTPases leverage their functions...
ORGANISM(S): Homo sapiens (Human) 
2026-03-09 | PXD068314 | Pride
Ribosome biogenesis Gtpase Ribosomal protein Gtpbp4 Gnl2 Pre-rrna processing Gnl3 Translation 
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