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ProteomicsProteomics-Based Retinal Target Engagement Analysis and Retina-Targeted Delivery of 17β-Estradiol by the DHED Prodrug in Males. Katalin Prokai-Tatrai,* Khadiza Zaman, Vien Nguyen, Daniel L. De La Cruz and Laszlo Prokai
We examined the impact of 17β-estradiol (E2) eye drops on the modulation of the proteome pro-file in the male rat retina. With discovery-driven proteomics, we have identified proteins that were regulated by the treatment. These proteins were assembled to several bioinformatics-based networks implica...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-11-04 | PXD027902 | Pride
Prote-omics Isotope-dilution Targeted proteomics Dhed prodrug Neuroprotection  lc-ms/ms 17β-estradiol  e2  eye drops  male retina  parallel-reaction monitoring 
ProteomicsProteomics Complementation of the Rat Uterotrophic Assay for Estrogenic Endocrine Disruptors: A Roadmap of Advancing High Resolution Mass Spectrometry-based Shotgun Survey to Targeted Biomarker Quantifications Authors: Laszlo Prokai *, Fatima Rahlouni, Khadiza Zaman,Vien Nguyen, and Katalin Prokai-Tatrai
The widely used rat uterotrophic assay to assess known and potential estrogenic compounds only considers uterine weight gain as endpoint measurement. To complement this method with an advanced technology that reveals molecular targets, we analyzed changes in protein expression using label-free quant...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-04-23 | PXD023273 | Pride
Strogen-regulated proteins  rat  uterus  liquid chromatography–mass spectrometry quantitative label-free proteomics  targeted pathway analysis  protein networks  endocrine disruption 
ProteomicsRetina-Targeted 17β-Estradiol by the DHED Prodrug Rescues Visual Function and Actuates Neuroprotective Protein Net-works after Optic Nerve Crush in a Rat Model of Surgical Menopause. Katalin Prokai-Tatrai, Khadiza Zaman, Ammar Kapic, Kelleigh Hogan , Gabriela Sanchez-Rodriguez, Anna E. Silverio Vien Nguyen , Laszlo Prokai and Andrew J. Feola
The association between 17β-estradiol (E2) deprivation, seen in menopause and a risk for devel-oping glaucoma, has been shown. Thus, exogenous supplementation of E2 may protect against retinal ganglion cell (RGC) degradation and vision loss. Here, we investigated the utility of topical 10β,17β-dihyd...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-05-07 | PXD059520 | Pride
Bioinformatics  dhed  estrogen  glaucoma  menopause  optic nerve crush  optomotor response  protein–protein interaction networks  quantitative label-free retina proteomics 
ProteomicsComparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action Prajita Pandey 1,‡, Khadiza Zaman 2, ‡, Laszlo Prokai 2 , and Vladimir Shulaev 1,*
The early signaling events involved in oxidant recognition and triggering of oxidant-specific defense mechanisms to counteract oxidative stress still remains largely elusive. Our discovery driven comparative proteomics analysis revealed unique early signaling response of yeast Saccharomyces cerevisi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-01-04 | PXD022685 | Pride
Oxidative stress  reactive oxygen species  antioxidants  global untargeted proteomics  tor signaling  ran signaling  mapk cascade 
Proteomics17β-Estradiol Delivered in Eye Drops: A Proteomics Study Focusing on the Rat Retina
Five ovariectomized (OVX) Brown Norway rats (Charles Rivers Laboratories, Wilmington, DE, USA) weighing 200-250 g received 10 µL of 17β-estradiol (E2) eye drops once daily in both eyes for three weeks [20]. The eye drops contained 0.1% (w/v) E2 in saline vehicle containing 20% (w/v) 2-hydroxypropyl-...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-05-27 | PXD010851 | Pride
Retina Datadependent acquisition Rat Label-free Estradiol 
ProteomicsProteomics-Based Identification of Retinal Protein Networks Impacted by Elevated Intraocular Pressure in the Hypertonic Saline Injection Model of Experimental Glaucoma
Elevated intraocular pressure is considered a major cause of glaucomatous retinal neurodegeneration. To facilitate a better understanding of the underlying molecular processes and mechanisms, we report a study focusing on alterations of the retina proteome by induced ocular hypertension in a rat mod...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-10-24 | PXD042729 | Pride
Retina proteomics  glaucoma  ocular hypertension  brown norway rat model  bioinformatics  pathway analysis  protein networks 
ProteomicsZebrafish Deyolking Survey - A Survey of the Impact of Deyolking on Biological Processes Covered by Shotgun Proteomic Analyses of Zebrafish Embryos
Deyolking, the removal of the most abundant protein from the zebrafish (Danio rerio) embryo, is a common technique for in-depth exploration of proteome-level changes in vivo due to various environmental stressors or pharmacological impacts during embryonic stage of development. However, the effect o...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2015-10-14 | PXD002124 | Pride
Bioinformatics Label-free proteomics Zebrafish Deyolking 
ProteomicsPG-regulated Synechocystis sp. PCC6803
Cells from the Synechocystis sp. PCC6803 strain were used to identify phosphatidylglycerol-regulated proteins by label-free quantitative shotgun proteomics combining SDS-PAGE prefractionation and data-dependent LC–MS/MS. Acquired data was searched against a composite protein sequence database of cya...
ORGANISM(S): Synechocystis sp. Synechocystis sp. PCC 6803 
2014-03-06 | PXD000363 | Pride
Quantitative label-free proteomics Cyanobacteria Phosphatidylglycerol Gelc-ms/ms Synechocystis sp. pcc6803 
ProteomicsVHL Cancer - Proteomic analysis of signaling network regulation in renal cell carcinomas with differential hypoxia-inducible factor-2α expression
The loss of von Hippel-Lindau (VHL) protein function leads to highly vascular renal tumors characterized by an aggressive course of disease and refractoriness to chemotherapy and radiotherapy. Loss of VHL in renal tumors also differs from tumors of other organs in that the oncogenic cascade is medi...
ORGANISM(S): Homo sapiens (Human) 
2013-08-12 | PXD000335 | Pride
Human Vhl Cancer Hif-2a Lc-ms/ms 
ProteomicsIdentification of Estrogen-Responsive Proteins in the Mouse Seminal Vesicle by Mass Spectrometry-Based Proteomics
Although estrogenic compounds promise therapeutic potential in treating various conditions, concerns regarding their endocrine-disrupting effects have been raised Current methodologies for screening estrogenicity in rodent models are limited to the female-specific uterotrophic bioassay. Studies have...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD056329 | Pride
7β-estradiol  male reproductive system  mouse seminal vesicle  therapeutic effects  endocrine disruption  proteomics  bioinformatics  biomarker discovery 
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