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GenomicsIntricate structure of the interphase chromocenter revealed by proximity based biotinylation
Intricate structure of the interphase chromocenter revealed by proximity based biotinylation
PRJNA564796 | ENA
GenomicsIntricate structure of the interphase chromocenter revealed by proximity based biotinylation
During interphase centromeres often coalesce into a small number of chromocenters, which can be visualized as distinct, DAPI dense nuclear domains. Intact chromocenters play a major role in maintaining genome stability as they stabilize the transcriptionally silent state of repetitive DNA while ensu...
ORGANISM(S): Drosophila melanogaster 
2020-01-01 | GSE137194 | GEO
ProteomicsThiol-cleavable Biotin for Chemical and Enzymatic Biotinylation and its Application to Mitochondrial TurboID Proteomics
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-beads digestion in numerous biological applications. However, the popular on-beads digestion method faces major challenges of streptavidin contamination, the lost information of bioti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-21 | MSV000087256 | MassIVE
UnknownA multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation.
Not available
S-EPMC7192626 | biostudies-literature
ProteomicsControl of TurboID-dependent biotinylation intensity in proximity ligation screens
Proximity biotinylation screens are a widely used strategy for the unbiased identification of interacting or vicinal proteins. The latest generation biotin ligase TurboID has broadened the range of potential applications, as this ligase promotes an intense and faster biotinylation, even in subcellul...
ORGANISM(S): Homo sapiens (Human) 
2023-03-29 | PXD040302 | Pride
Biotinylation Proximity ligation screens Protein-protein interactions Proteomics 
UnknownIdentification of potential nucleomodulins of Mycoplasma bovis by direct biotinylation and proximity-based biotinylation approaches.
Not available
S-EPMC11263210 | biostudies-literature
ProteomicsBIOSITe: A novel method for direct detection of site specific biotinylation
Proximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family protei...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-12-19 | PXD007862 | Pride
Bioid Proximity dependent biotinylation Bcr abl Mass spectrometry Ip Peptide Biotinylation Biosite 
UnknownCF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis.
Not available
S-EPMC9217950 | biostudies-literature
UnknownA proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.
Not available
S-EPMC8748630 | biostudies-literature
ProteomicsLysosomal damage proteomics in HeLa cells
To identify ubiquitylation targets following lysosomal damage in HeLa cells treated with LLOMe we performed quantitative ubiquitin-remmnant (diGly) profilig coupled to mass spectrometry (MS). In addition, we performed APEX2-based proximity biotinylation followed by MS analysis to identify proximity ...
ORGANISM(S): Homo sapiens (Human) 
2022-06-23 | PXD029926 | Pride
Apex2 Lysosome Proxiity biotinylation Llome Digly proteomics Ubiquitin Cnn2 
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