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ProteomicsArabidopsis thaliana DOG1 pull-downs from seed tissues
Functionally complemented dog1-1 mutant with a YFP-DOG1 fusion protein under the control of its native promoter were used as starting material for in vivo pull-down of YFP: DOG1 and its interacting proteins from seed tissues in native conditions. DOG1 interacting proteins were identified by mass spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-07-07 | PXD006347 | Pride
Arabidopsis thaliana Dormancy Dog1 Seed Germination 
ProteomicsControl pull-downs testing the effect of histone native chemical ligation on protein binding to di-nucleosomes
Here we examined whether histones H3 and H4 produced using native chemical ligation reaction affect protein binding to di-nucleosomes. To test this we performed a set of affinity purification pull-downs using di-nucleosomes containing the following histones H3 and H4: 1. ligated unmodified histone H...
ORGANISM(S): Homo sapiens (Human) 
2023-12-10 | PXD042630 | Pride
Nucleosome pull-down Native chemical ligation 
ProteomicsArabidopsis thaliana anti-all-WRKY pull-downs from whole seedlings
The experiments were performed to monitor dynamics in WRKY transcription factor abundancies upon treatment with flg22, a peptide derived from the bacterial flagella. Mock-treated or WT seedlings treated for 2 h with flg22 were used to prepare crude nuclear lysates. Then pull downs were performed wit...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-07-30 | PXD008971 | Pride
Wrky Arabidopsis Pull-down Flg22 
ProteomicsAnalysis of ZNF827 Halotag pulldown for a manuscript titled ‘ZNF827 is a single-stranded DNA binding protein that regulates the ATR-CHK1 DNA damage response pathway’
Mass spectrometry analysis of ZNF827 Halotag pulldown ZNF827 pull-downs were performed in HEK 293T cells overexpressing HaloTag ZNF827 or empty vector control using the HaloTag® Protein Pull-Down and Labelling System (Promega) according to the manufacturer’s protocol. Pull-downs were subjected mass ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-23 | PXD050191 | Pride
Atr Ssdna Znf827 Dna damage response 
GenomicsReciprocal interactions of human C10orf12 and C17orf96 with PRC2 revealed by BioTAP-XL crosslinking and affinity purification
Short-read sequencing of DNA fractions of the BioTAP-XL pull-downs on human EZH2 and interacting proteins.
ORGANISM(S): Homo sapiens 
2014-02-01 | GSE53495 | GEO
ProteomicsGPIF constrains Polycomb Repressive Complex 2 activity in germ cells
The project aimed at identifying the cofactors regulating Polycomb complex PRC2 enzymatic activity in gonads. We used a knock-in mouse model where the enzymatic subunit of PRC2 (either EZH2 or EZH1) is tagged (Flag-tag) to purify PRC2 from mouse adult testis. This experiment revealed the existence o...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-07-22 | PXD012354 | Pride
Histone Chromatin Methylation Polycomb. 
ProteomicsThe interactome of Zbtb2 in mouse embryonic stem cells
Zbtb2 is a transcription factor that plays a role in mouse embryonic stem cell (mESC) differentiation. We pulled-down endogenously tagged Zbtb2-FLAG in mESCs and found a complex interactome. We characterized the Zbtb2 complex further by co-pull-downs of overexpressed Zbtb2-AVI, after mutation of ind...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-14 | PXD025520 | Pride
Zbtb2 interactome Development Mouse embryonic stem cells 
TranscriptomicsThe miRNA miR-139-5p is a regulator of metastatic pathways in breast cancer.
Whole transcriptome Identification of direct targets of miR-139-5p using biotinylated pull-downs found that this miRNA has roles in breast cancer invasion and migration.
ORGANISM(S): Homo sapiens 
2013-12-01 | GSE40411 | GEO
ProteomicsMass spectrometry analysis of estrogen receptor α -interacting proteins.
Quantitative mass spectrometry analysis using tandem mass tags (TMT) labelling of estrogen receptor α (ERα) pull downs. Co-immunoprecipitated proteins using a anti Erα antibody were compared quantitatively with material recovered in a mock to identify ERα-interacting proteins.
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD025018 | Pride
Estrogen receptor Mrna binding Tmt Immunoprecipitation 
ProteomicsSelective Arylation of Selenocysteine of Thioredoxin Reductase 1 by an Organogold Compound: Expanding the Tool-Box of Metal-Templated Reactions in Cancer Cells – pull-down
Cyclometalated gold(III) complexes have been reported to template C-S cross-coupling reactions in a biological environment and acting as modifies of cysteine residues. To broaden the scope of organogold complexes for covalent protein post-translational modification in cancer cells, an oxime-containi...
ORGANISM(S): Homo sapiens (Human) 
2025-04-22 | PXD044971 | Pride
Arylation ∙ cancer ∙ chemoproteomics ∙ gold ∙ metals in medicine ∙ selenoprotein ∙ thioredoxin reductase 1 
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