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ProteomicsProteome profiling of cultivated Echerichia coli isolates from Crohn's disease patients
One of the dysbioses often observed in CD patients is an increased abundance of Escherichia coli (10-100 fold compared to healthy individuals) [Gevers et al., 2014]. E. coli isolates collected from CD and healthy patients were cultivated on LB medium at aerobic conditions up to medium log phase and...
ORGANISM(S): Escherichia coli 
2019-08-15 | PXD010920 | Pride
Crohn's disease Hplc-ms/ms Proteome E. coli 
ProteomicsHuman plasma proteins detected by HPLC-MS/MS after blood incubation with E. coli treated/not treated with mucin
Whole human blood incubated with E. coli isolate with or without bound mucin. Plasma was obtained and the effect of mucin bound to E. coli on plasma proteins content was analysed by HPLC-MS-MS.
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD010496 | Pride
Plasma Mucin E. coli 
ProteomicsMucin-binding proteins of E. coli membrane
Proteins from E. coli outer membrane were extracted with carbonate method, separated in 2D-PAGE and transferred onto nitrocellulose membrane. After incubation with flourescent-labelled mucin, mucin-binding protein spots were cut out, subjected to trypsinolysis and identified by protein analysis wit...
ORGANISM(S): Escherichia coli 
2019-12-16 | PXD010472 | Pride
Escherichia coli Hplc-ms-ms Mucin binding Membrane 
ProteomicsSpecial interactions of Bacteroides fragilis toxin with outer membrane vesicles reveal new mechanism of its secretion and delivery
Here we present MS1 spectra of nontoxigenic and toxigenic BF cells membrane extract, complemented by MS/MS spectra of separate parent ions in positive and negative mode --------- MS1 total spectra BFplus and BFminus in negative and positive mode 2015-09-04-pos-bminus-baselinel-1.d - MS1 acquisition...
ORGANISM(S): Bacteroides Fragilis 638r (ncbitaxon:862962) 
2016-12-07 | MSV000080382 | MassIVE
MetabolomicsGNPS - Special interactions of Bacteroides fragilis toxin with outer membrane vesicles reveal new mechanism of its secretion and delivery
Here we present MS1 spectra of nontoxigenic and toxigenic BF cells membrane extract, complemented by MS/MS spectra of separate parent ions in positive and negative mode --------- MS1 total spectra BFplus and BFminus in negative and positive mode 2015-09-04-pos-bminus-baselinel-1.d - MS1 acquisition...
ORGANISM(S): Bacteroides Fragilis 638r (ncbitaxon:862962) 
2016-12-07 | MSV000080382 | GNPS
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