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ProteomicsRapiGest Precipitation Depends on Peptide Concentration
The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. RapiGest consists of a hydrophilic head and a hydrophobic tail, which can be separated by acid hydrolysis. This allows for removal of ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Gallus gallus (Chicken) 
2021-09-07 | PXD025982 | Pride
In-solution digestion Lc-ms Rapigest Detergent Precipitation 
ProteomicsEvaluation of the technical reproducibility of serum proteome sample preparation methods to support clinical proteomics applications
Evaluation of the efficiency and reproducibility of sample prepration methods using iST-BCT kit from PreOmics or SMART Trypsin Kit (Thermo Fisher) with in-house Rapigest protocol in human serum samples prior to mass spectrometry analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-04-07 | PXD051002 | Pride
Serum  proteomics  biomarker  mass spectrometry  rapigest sf  ist-bct  smart digest trypsin 
ProteomicsEvaluation of two methods for LS-MS/MS proteomic analysis of formalin fixed and paraffin embedded tissues
The proteomics of archival samples has advanced significantly during the last two decades, making possible the use of vast FFPE tissue archives in biomarker studies. However, due to the limited data regarding comparison of the performance of different protocols, there is currently no consensus on th...
ORGANISM(S): Homo sapiens (Human) 
2021-01-15 | PXD021074 | Pride
Ffpe Rapigest Proteomics Fasp Protein extraction Biomarker Lc-ms/ms 
ProteomicsReproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization
Four single consecutive murine kidney tissue specimens were prepared with the direct trypsinization with RapiGest (DTR) approach or with the filter aided sample preparation (FASP) protocol using both 10k and 30k filter devices and analyzed by label-free, quantitative liquid chromatography – tandem m...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2018-08-15 | PXD006401 | Pride
Shotgun proteomics Archival tissue Rapigest Ffpe Fasp Biomarker discovery Label-free quantitation 
ProteomicsImproved protease digestion conditions for membrane protein detection
This work presents improved protease digestion conditions for membrane protein detection. Two improved conditions, 0.01% SDS or the combination of 10% MeOH and 0.01% RapiGest, were chosen and applied to proteins from Escherichia coli. The application of both improved conditions to a membrane protein...
ORGANISM(S): Escherichia coli 
2015-04-28 | PXD001824 | Pride
Proteomics Membrane protein E. coli 
UnknownComparison between chaotropic and detergent-based sample preparation workflow in tendon for mass spectrometry analysis.
Not available
S-EPMC5575552 | biostudies-literature
UnknownImproved protease digestion conditions for membrane protein detection.
Not available
S-EPMC4739822 | biostudies-literature
ProteomicsLC-MS/MS profiling of sample processing optimization of formalin-fixed, paraffin embedded tissues
Despite a clinical, economic, and regulatory imperative to develop companion diagnostics, precious few new biomarkers have been successfully translated into clinical use, due in part to inadequate protein assay technologies to support large-scale testing of hundreds of candidate biomarkers in formal...
ORGANISM(S): Homo sapiens (Human) 
2016-07-25 | PXD003770 | Pride
Human Ffpe Breast cancer tumor tissue Velos 
UnknownComparative evaluation of two methods for LC-MS/MS proteomic analysis of formalin fixed and paraffin embedded tissues.
Not available
S-EPMC7900714 | biostudies-literature
UnknownEvaluation of Fast and Sensitive Proteome Profiling of FF and FFPE Kidney Patient Tissues.
Not available
S-EPMC8838561 | biostudies-literature
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