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ProteomicsThe GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform
Real-time database searching allows for simpler and automated proteomics workflows as it eliminates technical bottlenecks in high throughput experiments. Most importantly, it enables results dependent acquisition (RDA) where search results can be used to guide data acquisition during acquisition. Th...
ORGANISM(S): Homo sapiens (Human) 
2023-03-13 | PXD040716 | Pride
Paser Glycoproteomics Glycopaser Results dependent acquisition (rda) Real-time search 
ProteomicsStradard protein and yeast top-down data
We developed a novel dissociation strategy in the data acquisition process, the Mesh strategy to dissociate multiple charge states of one proteoform with stepped HCD (high collision energy dissociation). We proved that the ‘Mesh’ strategy could generate fragments with improved sequence coverage and...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-06-09 | PXD022941 | Pride
Stepped hcd Instrument control Real-time Mesh Top-down 
ProteomicsReal-time library search assisted identification of cross-links increases resolution for structural modeling and interactome mapping
Cross-linking mass spectrometry (XL-MS) is a universal tool of molecular and structural biology for probing structural dynamics and protein-protein interactions in vitro and in vivo. Although cross-linked peptides are naturally less abundant than their unlinked counterparts, recent experimental adva...
ORGANISM(S): Escherichia coli 
2023-03-20 | PXD037652 | Pride
Rtls E coli Cross-linking Diagnostic peaks Xl-ms Phox 
TranscriptomicsReal-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation
Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI ...
ORGANISM(S): Homo sapiens 
2014-06-01 | E-GEOD-51677 | biostudies-arrayexpress
ProteomicsReal-time molecular diagnosis of tumors using water-assisted laser desorption/ionization mass spectrometry technology
This dataset contains MS imaging raw data as well as the SCiLS file used to confirm the regions in dog sarcoma biopsies classified by the SpiderMass system after analysis of the consecutive tissue section. All the tissues were gathered during dog sarcoma surgeries, snap-frozen and analysed later in ...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2019-04-12 | PXD010990 | Pride
Molecular signatures Sarcoma Real-time Cancer Non-invasive Diagnostic Laser microprobe Spidermass Surgery Ambient ionization mass spectrometry 
UnknownQuality Control Analysis in Real-time (QC-ART): A Tool for Real-time Quality Control Assessment of Mass Spectrometry-based Proteomics Data.
Not available
S-EPMC6126382 | biostudies-literature
UnknownA robust, low-cost instrument for real-time colorimetric isothermal nucleic acid amplification
Not available
S-EPMC9524685 | biostudies-literature
GenomicsReal-time quantitative PCR analysis of Nannochloropsis gaditana
Real-time quantitative PCR analysis of Nannochloropsis gaditana
PRJNA864746 | ENA
UnknownNormalized quantification of human cytomegalovirus DNA by competitive real-time PCR on the LightCycler instrument.
Not available
S-EPMC154596 | biostudies-literature
TranscriptomicsReal-time quantitative PCR analysis of human malignant pleural mesothelioma tissue specimens
BACKGROUND: Malignant pleural mesothelioma (MPM) is difficult to distinguish from reactive mesothelial proliferations (RMPs). MicroRNAs (miRNAs) are small non-coding RNA-strands (~22 nucleotides) that post-transcriptionally regulate gene-expression. Studies have shown that miRNAs are potential diagn...
ORGANISM(S): Homo sapiens 
2014-01-25 | E-GEOD-54394 | biostudies-arrayexpress
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