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ProteomicsRecombinant Protein Spectral Library (rPSL) DIA-MS Method Improves Identification and Quantification of Low-Abundance Cancer-Associated and Kynurenine Pathway Proteins
Data-independent acquisition mass spectrometry (DIA-MS) is a powerful tool for quantitative proteomics, but a well-constructed reference spectral library is crucial to optimize DIA analysis, particularly for low-abundance proteins. In this study, we evaluate the efficacy of a recombinant protein spe...
ORGANISM(S): Homo sapiens (Human) 
2025-05-15 | PXD061696 | Pride
Snap frozen tissues Rpsl Dia-ms Cancer- associated proteins Cell lysates Kynurenine pathway proteins Recombinant protein spectral library 
ProteomicsRecombinant SWATH library for identification of low abundant human plasma proteins
Recombinant SWATH library for identification of low abundant human plasma proteins
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-04 | MSV000086411 | MassIVE
UnknownThe NISTmAb tryptic peptide spectral library for monoclonal antibody characterization.
Not available
S-EPMC5916550 | biostudies-literature
ProteomicsEvaluation of the False Discovery Rate in Library-free Search by DIA-NN Using in vitro Human Proteome
In recent years, deep learning-based in silico spectral libraries have gained increasing attention. Several data-independent acquisition (DIA) software tools have integrated this feature, known as library-free search, making DIA analysis more accessible. However, controlling the false discovery rate...
ORGANISM(S): Cellular Organisms 
2025-07-23 | PXD056519 | JPOST Repository
ProteomicsGlycoproteomics of recombinant Measles Virus F from Drosophila S2 cells
Recombinant soluble ectodomains of a prefusion-stabilized MeV F-protein were analysed by bottom-up proteomics to profile the N-glycans.
ORGANISM(S): Measles morbillivirus 
2025-01-02 | PXD050366 | Pride
Measles virus Glycoproteomics 
ProteomicsVariation of Site-Specific Glycosylation Profiles of Recombinant Influenza Glycoproteins
Recombinant proteins are of great interest in glycobiology and proteomics, known especially for their reproducibility and accessibility. However, variation in glycosylation among recombinant glycoproteins is not well understood and may depend on numerous conditions in the biomanufacturing process. I...
ORGANISM(S): Influenza A virus (A/California/04/2009(H1N1)) Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) Influenza A virus (A/Japan/305/1957(H2N2)) Influenza A virus (A/Netherlands/219/2003(H7N7)) Influenza A virus (A/Hong Kong/483/1997(H5N1)) Influenza A virus (A/New Caledonia/20/1999(H3N2)) Influenza A virus (A/Arizona/13/2008(H1N1)) Influenza A virus (A/Hong Kong/485197/2014(H3N2)) 
2024-08-13 | PXD042062 | Pride
Glycosylation Influenza a Site-specific Hemagglutinin 
ProteomicsGlycoproteomics profiling of recombinant SARS-CoV-2 stabilized Spike construct
N-linked glycans of recombinant SARS-CoV-2 Spike protein with stabilizing mutations were profiled by LC-MS/MS.
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 
2021-07-28 | PXD025554 | Pride
Sars-cov-2 Glycoproteomics Covid-19 
ProteomicsDevelopment of a parallel reaction monitoring (PRM) mass spectrometry-assay for the detection of SARS-CoV-2 spike glycoprotein and nucleoprotein
There is an urgent need for robust and high-throughput methods for SARS-CoV-2 detection in suspected pa-tient samples to facilitate disease management, surveillance, and control. Although nucleic acid detection methods such as RT-PCR are the gold standard, during the current pandemic the deployment ...
ORGANISM(S): SARS coronavirus WHU 
2022-01-12 | PXD021080 | Pride
Skyline Nucleoprotein Qexactive hf-x Parallel reaction monitoring Sars-cov-2 Heavy standard peptides Spike protein 
ProteomicsEnhanced Proteomics Analysis with a Novel Recombinant Chymotrypsin Analogue En-gineered for High Cleavage Specificity
Chymotrypsin is widely used in shotgun proteomics owing to its orthogonal cleavage specificity relative to trypsin, which enhances sequence coverage of hydrophobic protein regions. However, commercial preparations often display variable cleavage specificity, trypsin contamination, and elevated misse...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD072165 | Pride
Chymotrypsin Missed cleavages Sample preparation Proteomics Protein digestion 
ProteomicsCellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells
Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo concentration dependent dimerization in vitro. To test whether the cellular concentration of PKD1 is in the range of the dissociation constant obtained, the expression level of PKD1 was investigated by PRM. To this end...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2019-08-19 | PXD013232 | Pride
Pkd1 Ins-1 Protein kinase d Hek293t 
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