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ProteomicsCryptococccus Rav1-mCherry LC-MS/MS
we performed in vivo pull-down experiment using the rav1Δ::RAV1-mCherry strain and RFP-trap agarose beads to precipitate Rav1-mCherry protein and its interacting partners.
ORGANISM(S): Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) 
2023-10-02 | PXD042074 | Pride
Rfp trap agarose beads Cryptococcus Rav1-mcherry Lc-ms/ms 
ProteomicsThe Legionella protein VpdC is a ubiquitin-activated phospholipase effector
The goal of this project was to identify the host factor(s) required for allosteric activation of the phospholipase VpdC from the intracellular pathogen Legionella pneumophila. For that reason, we performed co-precipitation analyses from lysate of transiently transfected HEK293T cells producing eith...
ORGANISM(S): Legionella pneumophila Legionella pneumophila 
2023-06-12 | PXD037059 | Pride
Host-pathogen interaction  intracellular pathogen  legionnaires' disease  translocated effector  host cell 
ProteomicsIdentification of potential interactors of NKS1 and QUA1
Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae are enriched in pectin polysaccharides, specifically homogalacturonan (HG). Pectins are m...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-03-20 | PXD047727 | Pride
Arabidopsis thaliana  na+ and k+-sensitive 1 (nks1)/elmo4  qua1  pectin 
ProteomicsQuantitative analysis of septin Cdc10 & Cdc3-associated proteome during stress response in the fungal pathogen Cryptococcus neoformans
Cryptococcus neoformans is a type of pathogenic fungi that can cause infections in people with weakened immune systems. This fungal pathogen can survive in the body by growing at the host's body temperature (37\'b0C), forming a carbohydrate capsule, and producing melanin. When Cryptococcus neoforman...
ORGANISM(S): Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) 
2024-10-28 | PXD052407 | Pride
Cryptococcus neoformans Septin Co-ip 
ProteomicsFungus-host protein-protein interactions between Drechmeria coniospora and Caenorhabditis elegans
Label-free quantitative affinity purification experiments were performed to identify the host protein binders of D. coniospora fungal virulence factors in C. elegans.
ORGANISM(S): Caenorhabditis elegans 
2020-11-20 | PXD021929 | Pride
Innate immunity Ap-ms Ppi C. elegans Drechmeria coniospora Fungus-host interaction 
ProteomicsThe Drosophila Epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour.
During Drosophila epithelial development, dynamic signalling filopodia (cytonemes) establish direct contacts between distant cells to facilitate the formation of the Hedgehog signalling gradient. However, not much is known about how cytonemes are regulated. In this study, we show that cytoneme dynam...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2025-05-06 | PXD040793 | Pride
Ihog Development. Hedgehog signalling Egfr Cytonemes 
ProteomicsRepurposing Herpesvirus Protein ORF57 to Inhibit TDP-43 Aggregation
The integrated stress response (ISR) is a major element of translation control. The ISR is beneficial in responding to acute stresses, but in human disease a chronic, persistent stress response can contribute to the disease process. Many studies manipulate the ISR by targeting just one protein. Howe...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD039246 | Pride
Translational stress response Alzheimer’s disease Als Protein aggregation Tdp-43 
ProteomicsMistranslation from endogenous tRNA variants in human pan-genome cell lines
Errors in protein synthesis are thought to be rare, yet cells representing the diversity of life tolerate greatly elevated levels of error. One source of translation error in humans are anticodon mutations in transfer RNAs (tRNAs). We found a tRNA Ser AGA -2-3 variant (G35A) that occurs in 2% of the...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD064886 | Pride
Mistranslation Trna variants Translation error 
ProteomicsCryptococcus Wcp1-mCherry Wcp1-FLAG AP–MS
This study aims to identify Wcp1-interacting proteins in Cryptococcus neoformans under different pH conditions. Wcp1 is a WD40–cyclophilin fusion protein implicated in adaptation to host-associated stresses, including CO2, temperature, and acidic pH. Affinity purification coupled with liquid chrom...
ORGANISM(S): Fungi Cryptococcus neoformans H99-YPD_1 
2026-05-16 | PXD077146 | Pride
Wcp1 Cryptococcus Ph 
ProteomicsRegulation of Drosophila brain development and organ growth by the Minibrain/Rala signaling network
Using affinity purification-mass spectrometry (AP-MS), we identified the novel Mnb binding partners Ral interacting protein (Rlip) and RALBP1 associated Eps domain containing (Reps). Rlip and Reps physically and genetically interact with Mnb, and the three proteins may form a ternary complex. Mnb ph...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-09-09 | PXD052378 | Pride
Embryo Drosophila Minibrain 
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