Sort   by:  
 Page size 
ProteomicsPulldown of RNA binding protein using SARS CoV 2 NSP1 RNA
RNA sensing is critical for RLR activation and downstream signaling. To identify molecules that are involved in modulating viral RNA recognition, we utilized biotin-labeled 60 nt single-stranded RNA (ssRNA) from SARS-CoV-2 NSP1 coding region (NSP1 ssRNA) to pull down proteins from RAW264.7 cells. Th...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-08 | PXD064808 | Pride
Pulldown  rna binding protein  nsp1 rna  sars-cov-2 
ProteomicstRF-24 RNA pulldown in KYSE150 and KYSE30
To delineate the oncogenic mechanism of tRF-24 in ESCC, we performed RNA pull-down assays with biotinylated tRF-24 and antisense control, followed by mass spectrometry (MS) analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-12-01 | PXD063163 | Pride
Human Kyse30 Esophagus Rna-pulldown Kyse150 
UnknownMultiple Oligo assisted RNA Pulldown via Hybridization followed by Mass Spectrometry (MORPH-MS) for exploring the RNA-Protein interactions.
Not available
S-EPMC10730167 | biostudies-literature
ProteomicsLinc-ADAIN Pulldown assay in Human Adipocytes.
Description: a biotinylated pulldown assay of linc-ADAIN, lincRNA vs Antisense Linc-ADAIN in primary human ASC adipocytes coupled to mass spectrometry (MS).
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD051388 | Pride
Pulldown assay Linc-rna protein interaction Adipocytes Lincrna Linc-adain 
ProteomicsUtilization of Stability-based Proteomics for the Investigation of Structured RNA-protein Interactions
The stability of proteins from rates of oxidation (SPROX), thermal protein profiling (TPP) methods as well as triple helix pulldowns with LC-MS/MS readouts are used to identify the protein targets of three RNA ligands, the MALAT1 triple helix (TH), a viral stem loop (SL), and an unstructured RNA (Po...
ORGANISM(S): Homo sapiens (Human) 
2024-01-31 | PXD043878 | Pride
Thermodynamics Thermal denaturation Chemical denaturation Rna Rna-protein interactions Proteomics 
ProteomicsRNA antisense oligonucleotide pulldown coupled with mass spectrometry (MS) to identify C1QTNF1-AS1 protein interactors
We used C1QTNF1-AS1 antisense oligonucleotide pulldown coupled with MS to identify protein interactors of lncRNA C1QTNF1-AS1 in human HCT116 cells. Five antisense oligonucleotides against C1QTNF1-AS1 and luciferase (negative control) were used for the pulldown. Three independent oligonucleotides (C...
ORGANISM(S): Homo sapiens (Human) 
2026-03-12 | PXD059546 | Pride
Antisense oligo pulldown  rna interactome  lc-ms/ms  label-free quantification 
Proteomicssin-lncRNA interactors in senescent fibroblasts MS
sin-lncRNA is a noncoding RNA molecule with a role in cellular senescence. In vitro RNA pulldown of biotynilated sin-lncRNA was performed to identify sin-lncRNA specific protein partners
ORGANISM(S): Homo sapiens (Human) 
2025-04-29 | PXD044718 | Pride
Human fibroblasts Lncrna Ois Lc/ms/ms Senescence 
TranscriptomicsChromatin immunoprecipitation of RNA binding protein CFM2 in Zea mays chloroplast extracts
Identification of target transcripts for the putative chloroplast RNA binding protein CFM2 in Zea mays. CFM2 was immunoprecipitated from a chloroplast extract. Chloroplast extracts were prepared from WT tissue. RNA from the pellet and from the supernatant for each pulldown was labelled with differen...
ORGANISM(S): Zea mays 
2006-09-01 | E-MEXP-743 | biostudies-arrayexpress
TranscriptomicsConserved functional antagonism between CELF and MBNL proteins regulates stem cell-specific alternative splicing and regeneration in planarians
Here, we analyze the RNA-binding preferences of the planarian smed-mbnl-like-2, smed-mbnl-1 (isoform X1), smed-bruli, smed-mbnl-1 (isoform Xins), and smed-mbnl-like-1 protein using RNAcompete. In the RNAcompete assay, a purified GST-tagged protein is incubated with an excess of RNA pool and bound RN...
ORGANISM(S): synthetic construct 
2016-08-01 | E-GEOD-75554 | biostudies-arrayexpress
TranscriptomicsThe crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation
Here, we analyze the RNA-binding preferences of several NHL containing RNA-binding proteins (BRAT, NCL-1, TRIM71, WECH, LIN-41, TRIM56, and MEI-P26) using RNAcompete. In the RNAcompete assay, purified GST-tagged RNA-binding protein is incubated with an excess of RNA pool and bound RNA from individua...
ORGANISM(S): synthetic construct 
2015-09-14 | E-GEOD-73000 | biostudies-arrayexpress
Sort   by:  
 Page size