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ProteomicsRetention time calibration for RP-HPLC at pH 9
The change in pH of the mobile phase drastically changes the retention mechanism of peptides, rendering currently available retention time prediction models useless. The aim of this study is to create a dataset of peptide retention times under basic (pH 9) HPLC conditions, allowing for the creation ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-13 | PXD064311 | Pride
Hela Basic ph Rp-hplc 
ProteomicsHuman follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation
Analysis of proteomic composition of human follicular fluid (hFF) has been proposed as a potential tool of oocyte quality evaluation. In order to develop an efficient method to investigate the hFF proteome and peptidome components, we applied a prefractionation scheme of hFF material consisting of ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-19 | PXD008073 | Pride
Human Follicular fluid Fractionation Swath-ms 
ProteomicsProteome analysis by charge state-selective separation of peptides: a multidimensional approach
Multidimensional peptide fractionation is widely used in proteomics to reduce the complexity of peptide mixtures prior to mass spectrometric analysis. Here, we describe the sequential use of strong cation exchange and reversed phase liquid chromatography in both basic and acidic pH buffers for separ...
ORGANISM(S): Mus musculus (Mouse) 
2013-11-27 | PXD000210 | Pride
Lc-msms Rp-hplc basic ph Mouse Scx 
ProteomicsMapping of transglutaminase 2 active sites of human salivary small basic proline-rich
Due to the distinctive features of oral mucosa, determination of the proteins involved in the formation of “oral protein pellicle” is demanding. The present study investigated the susceptibility of several human basic proline-rich peptides (bPRPs) named P-H, P-D, P-F, P-J, and II-2 as substrates of ...
ORGANISM(S): Homo sapiens (Human) 
2019-10-25 | PXD014658 | Pride
Bprps Saliva Transglutaminase 2 Human 
UnknownQuality-by-design based RP-HPLC analytical method for the estimation of quercetin in silver-metal organic frameworks (AgMOF): a Box-Behnken optimization approach.
Not available
S-EPMC12764978 | biostudies-literature
ProteomicsGenomics and proteomics of biofuel
Bacteria was grown at 30C in 3 different conditions, i.e. SYN (syngas and minimal medium- ATCC no 1789), AC (0.3% acetate in minimal medium- ATCC no 1789) and TSB (tryptic soy broth). After harvesting by centrifugation, O. carboxidovorans pellets (1g) were lysed in 100 mM Tris-Cl pH 8.0, 2% Triton X...
ORGANISM(S): Oligotropha carboxidovorans (Pseudomonas carboxydovorans) 
2012-05-23 | PRD000150 | Pride
UnknownBiochemical, pharmacological, and structural characterization of new basic PLA2 Bbil-TX from Bothriopsis bilineata snake venom.
Not available
S-EPMC3591176 | biostudies-literature
UnknownProduction and Antibacterial Activity of Atypical Siderophore from Pseudomonas sp. QCS59 Recovered from Harpachene schimperi.
Not available
S-EPMC11434927 | biostudies-literature
UnknownComparative stability study and aggregate analysis of Bevacizumab marketed formulations using advanced analytical techniques.
Not available
S-EPMC10558615 | biostudies-literature
UnknownOxidations of Benzhydrazide and Phenylacetic Hydrazide by Hexachloroiridate(IV): Reaction Mechanism and Structure-Reactivity Relationship.
Not available
S-EPMC7024218 | biostudies-literature
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