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ProteomicsProteomic survey of the Streptomyces coelicolor nucleoid
Nucleoid-associated proteins (NAPs) are small, highly abundant regulators with low sequence specificity and pleiotropic mutant phenotypes. They are involved in transcriptional and post-transcriptional control of gene expression, DNA protection/repair and nucleoid structuring. Discovery of the major ...
ORGANISM(S): Streptomyces coelicolor A3(2) 
2013-04-24 | PRD000736 | Pride
ProteomicsDetermination of Phosphorylation Sites in the DivIVA Protein of Streptomyces coelicolor by Targeted LC-MS/MS
The filamentous bacterium Streptomyces coelicolor modulates polar growth and branching by phosphorylating the cytoskeletal protein DivIVA. Previous MALDI-TOF analysis of DivIVA showed that a large 7.2 kDa tryptic peptide was multiply phosphorylated. To aid localization of the phosphorylation sites, ...
ORGANISM(S): Streptomyces coelicolor 
2013-08-05 | PXD000095 | Pride
Phosphopeptides Targeted lc-ms/ms Phosphorylation site localization Diviva Orbitrap Scaffoldptm Ascore 
ProteomicsGlycosyltransferases from oat (Avena) implicated in the acylation of avenacins
To study glycosyltransferases from oat, root proteins were extracted and separated gy 1D SDS gel electrophoresis. Bands were cut out, digested with trypsin, and the resulting peptides were analysed by LCMSMS on an Orbitrap mass sepctrometer. Raw data was processed with MaxQuant 1.3.0.5, and database...
ORGANISM(S): Avena strigosa (black oat) 
2013-04-24 | PXD000083 | Pride
Terpenes  saponin  glycosyltransferase  cereals 
ProteomicsPlasmid manipulation of bacterial behaviour through translational regulatory crosstalk
pQBR103 is a naturally occurring plasmid known to alter the behaviour of its host, Pseudomonas fluorescens. This plasmid encodes a predicted translational regulator, rsmQ which is a homologue of known global regulators within Pseudomonas. Within this study we compared Pseudomonas fluorescens SBW25 p...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) Bacteria 
2023-01-13 | PXD033640 | Pride
Tmt Rnaseq Translational regulator Pseudomonas fluorescens sbw25 Quantitative proteomics Plasmid 
ProteomicsAn integrative, multi-omics analysis of the Hfq regulon
The ability of bacteria to respond to environmental change is based on the exploration of sophisticated regulatory landscapes. While we can learn a great deal from reductive analyses of individual pathways, and global approaches to gene regulation, a deeper understanding of these complex signalling ...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) 
2017-10-18 | PXD005621 | Pride
Hfq Pseudomonas fluorescens Posttranscriptional regulation Multi-omics Proteomics Isobaric labelling 
ProteomicsSecond messenger control of mRNA translation by dynamic ribosome modification
Pseudomonas species employ complex, dynamic signalling networks to fine-tune responses to changing environments, with regulation taking place at the transcriptional, post-transcriptional and post-translational levels. Control of mRNA translation and hence protein abundance is a crucial element of th...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) 
2020-06-30 | PXD017233 | Pride
Signalling Pseudomonas Ribosomal proteins Cyclic-di-gmp Itraq proteome 
ProteomicsThe zebrafish Danio rerio sperm proteome by LC-MSMS
Fertilisation is based on the tight interaction between sperm and egg and both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the mechanisms involved during fertilisation and ...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2025-01-27 | PXD056057 | Pride
Fertility Proteomics Danio rerio Fertilisation Sperm function Sperm-egg interaction 
ProteomicsVAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin 2 regulation at Arabidopsis FLC
• Polycomb (PcG) regulation is crucial for development across eukaryotes, but how PcG targets are specified is still incompletely understood. The slow timescale of cold-induced Polycomb Repressive Complex 2 silencing during vernalization at Arabidopsis thaliana FLOWERING LOCUS C (FLC) provides an ex...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-13 | PXD036548 | Pride
Asap Arabidopsis Vernalisation Polycomb Transcription factor Chromatin regulation Prc1 Epigenetics Gene silencing Orbitrap fusion 
ProteomicsUncoupled activation and cyclisation in catmint reductive terpenoid biosynthesis
Terpenes synthases typically form complex molecular scaffolds by concerted activation and cyclization of linear starting materials in a single active site. We have determined that iridoid synthase, an atypical reductive terpene synthase, catalyses the activation of its substrate 8-oxogeranial into a...
ORGANISM(S): Nepeta mussinii 
2019-08-12 | PXD008704 | Pride
Lc-msms Catmint Proteomics Trichome Iridoid Nepetalactone 
ProteomicsHow legumes control nitrogen fixation by root nodule bacteria
Rhizobia are soil bacteria that can enter into complex symbiotic relationships with legumes, where rhizobia induce the formation of nodules on the plant root. Inside nodules, rhizobia differentiate into nitrogen-fixing bacteroids that reduce atmospheric nitrogen into ammonia, secreting it to the pla...
ORGANISM(S): Rhizobium leguminosarum bv. viciae 3841 
2021-09-09 | PXD019467 | Pride
15n labeling Protein Rhizobium Quantifiication Pea Bacteroid 
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