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ProteomicsProteomic survey of the Streptomyces coelicolor nucleoid
Nucleoid-associated proteins (NAPs) are small, highly abundant regulators with low sequence specificity and pleiotropic mutant phenotypes. They are involved in transcriptional and post-transcriptional control of gene expression, DNA protection/repair and nucleoid structuring. Discovery of the major ...
ORGANISM(S): Streptomyces coelicolor A3(2) 
2013-04-24 | PRD000736 | Pride
ProteomicsDetermination of Phosphorylation Sites in the DivIVA Protein of Streptomyces coelicolor by Targeted LC-MS/MS
The filamentous bacterium Streptomyces coelicolor modulates polar growth and branching by phosphorylating the cytoskeletal protein DivIVA. Previous MALDI-TOF analysis of DivIVA showed that a large 7.2 kDa tryptic peptide was multiply phosphorylated. To aid localization of the phosphorylation sites, ...
ORGANISM(S): Streptomyces coelicolor 
2013-08-05 | PXD000095 | Pride
Phosphopeptides Targeted lc-ms/ms Phosphorylation site localization Diviva Orbitrap Scaffoldptm Ascore 
ProteomicsGlycosyltransferases from oat (Avena) implicated in the acylation of avenacins
To study glycosyltransferases from oat, root proteins were extracted and separated gy 1D SDS gel electrophoresis. Bands were cut out, digested with trypsin, and the resulting peptides were analysed by LCMSMS on an Orbitrap mass sepctrometer. Raw data was processed with MaxQuant 1.3.0.5, and database...
ORGANISM(S): Avena strigosa (black oat) 
2013-04-24 | PXD000083 | Pride
Terpenes  saponin  glycosyltransferase  cereals 
ProteomicsPlasmid manipulation of bacterial behaviour through translational regulatory crosstalk
pQBR103 is a naturally occurring plasmid known to alter the behaviour of its host, Pseudomonas fluorescens. This plasmid encodes a predicted translational regulator, rsmQ which is a homologue of known global regulators within Pseudomonas. Within this study we compared Pseudomonas fluorescens SBW25 p...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) Bacteria 
2023-01-13 | PXD033640 | Pride
Tmt Rnaseq Translational regulator Pseudomonas fluorescens sbw25 Quantitative proteomics Plasmid 
ProteomicsAn integrative, multi-omics analysis of the Hfq regulon
The ability of bacteria to respond to environmental change is based on the exploration of sophisticated regulatory landscapes. While we can learn a great deal from reductive analyses of individual pathways, and global approaches to gene regulation, a deeper understanding of these complex signalling ...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) 
2017-10-18 | PXD005621 | Pride
Hfq Pseudomonas fluorescens Posttranscriptional regulation Multi-omics Proteomics Isobaric labelling 
ProteomicsBatR: A novel regulator of antibiotic tolerance in P. aeruginosa biofilms
Pseudomonas aeruginosa is a multidrug-resistant opportunistic human pathogen. Chronic infections are associated with biofilms, conferring resistance to antimicrobial agents and complicating treatment strategies. This study focuses on understanding the role of the uncharacterized gene PA3049, upregul...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2026-06-26 | PXD050995 | Pride
Proteomics Pseudomonas aeruginosa Antibiotic tolerance Biofilms 
ProteomicsA CPSF-like phosphatase module links transcription termination to chromatin silencing
Aiming to find the connection between RNA processing and chromatin remodelling, we found that a complex removing activating histone marks at FLC named FLD complex interacts with ARPF1, one of the homologs in Arabidopsis of the yeast Swd2. In this experiment, we used a FLAG-tagged version of the ARPF...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-13 | PXD049114 | Pride
Proteomics Rna-processing Swd2-like Chromatin Co-immunoprecipitatiion Cpsf 
ProteomicsBatR: A novel regulator of antibiotic tolerance in P. aeruginosa biofilms
Pseudomonas aeruginosa is a multidrug-resistant opportunistic human pathogen. Chronic infections are associated with biofilms, conferring resistance to antimicrobial agents and complicating treatment strategies. This study focuses on understanding the role of the uncharacterized gene PA3049, upregul...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2026-06-26 | PXD050997 | Pride
Proteomics Pseudomonas aeruginosa Antibiotic tolerance Biofilms 
ProteomicsSecond messenger control of mRNA translation by dynamic ribosome modification
Pseudomonas species employ complex, dynamic signalling networks to fine-tune responses to changing environments, with regulation taking place at the transcriptional, post-transcriptional and post-translational levels. Control of mRNA translation and hence protein abundance is a crucial element of th...
ORGANISM(S): Pseudomonas fluorescens (strain SBW25) 
2020-06-30 | PXD017233 | Pride
Signalling Pseudomonas Ribosomal proteins Cyclic-di-gmp Itraq proteome 
ProteomicsPost-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.
O-linked-β-N-Acetylglucosamine (O-GlcNAc) and O-fucose are two sugar-based post-translational modifications (PTMs) whose mechanistic role in plant signalling and transcriptional regulation is still largely unknown. Here, we investigated how two O-glycosyltransferase enzymes of Arabidopsis thaliana, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-22 | PXD043987 | Pride
Spy Mass spectrometry Spt O-glycosylation Gynoecium Sec 
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