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ProteomicsExonuclease-1 interactome and phosphorylation sites
To help shedding light on regulatory aspects of DNA repair and remodeling pathways in which EXO1 participates and to help revealing novel cellular processes in which EXO1 is involved, we set out to identify proteins interacting with EXO1. Both a small-scale approach and a comprehensive analysis of t...
ORGANISM(S): Homo sapiens (Human) 
2019-01-31 | PXD002780 | Pride
Human Exo1 Phosphorylation Affinity tag purification 
ProteomicsUbiquitination site analysis of eGFP by DARPin-based bioPROTACs analysed by LC-MS-MS
We investigated design principles of DARPin-based bioPROTACs targeting the model substrate GS-eGFP (eGFP with an n-terminal tag, necessary for proteasomal unfolding). Using a highly active E3 ligase domain from CHIP and nine well-characterized DARPins, we evaluated their ability to induce proteasoma...
ORGANISM(S): Homo sapiens (Human) 
2025-06-05 | PXD063877 | Pride
Ubiquitination Darpin Bioprotac Egfp 
ProteomicsDetermination of Phosphorylation Sites in the DivIVA Protein of Streptomyces coelicolor by Targeted LC-MS/MS
The filamentous bacterium Streptomyces coelicolor modulates polar growth and branching by phosphorylating the cytoskeletal protein DivIVA. Previous MALDI-TOF analysis of DivIVA showed that a large 7.2 kDa tryptic peptide was multiply phosphorylated. To aid localization of the phosphorylation sites, ...
ORGANISM(S): Streptomyces coelicolor 
2013-08-05 | PXD000095 | Pride
Phosphopeptides Targeted lc-ms/ms Phosphorylation site localization Diviva Orbitrap Scaffoldptm Ascore 
ProteomicsIntegration of time- and dose-dependent effects of salinity stress on the branchial proteome of euryhaline tilapia
Experiments were performed by Larken Root in the laboratory of Dietmar Kültz at UC Davis. Tilapia were acclimated at different rates from freshwater (FW) to elevated salinity. FW handling controls were included in parallel. Samples represent biological replicates, i.e. each sample was obtained f...
ORGANISM(S): Oreochromis Mossambicus (ncbitaxon:8127) 
2020-07-14 | MSV000085745 | MassIVE
ProteomicsPhospho-proteomic characterization of human hepatocellular carcinoma (HCC) tumor biopsies
We analyzed the phospho-proteome of tumor and matched non-tumor biopsies from 51 treatment-naive Hepatocellular carcinoma (HCC) patients by label-free DDA. Thereby we aim to find subgroups of patients characterized by specific pathway activation. Furthermore, we aim to find novel factors involved in...
ORGANISM(S): Homo sapiens (Human) 
2022-03-14 | PXD025836 | Pride
Human Qexactive hf Phospho-proteomics Hepatocellular carcinoma (hcc) Biopsies 
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