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ProteomicsProteomic analysis of the spatiotemporal based molecular kinetics of acute spinal cord injury identifies a time- and segment-specific window for effective tissue repair - IgG identification
Spinal cord injury (SCI) represents a major debilitating health issue with a direct socioeconomic burden on the public and private sectors worldwide. Although several studies have been conducted to identify the molecular progression of injury sequel due from the lesion site, still the exact underlyi...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-06-08 | PXD004247 | Pride
1d gel Mass spectrometry Igg Spinal cord injury 
ProteomicsMoving Forward Real-Time Proteomics Mass Spectrometry by Remote Infrared Matrix-Assisted Laser Desorption-Ionization
Novel development makes remote real-time analysis with possible translation to in-vivo a reality. Remote Infrared Matrix Assisted Laser Desorption Ionization (Remote IR MALDI) system with endogenous water as matrix becomes real and allows to envisage real-time proteomics to be performed in the in-vi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2018-04-20 | PXD008092 | Pride
Mass spectrometry • real-time analysis • remote ir maldi• proteins • charge state distribution 
ProteomicsON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS
Hydrogen/deuterium exchange mass spectrometric methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exc...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-08-15 | PXD004630 | Pride
Tissue Mass spectrometry In situ analysis H/d exchange 
ProteomicsInhibition of Furin in CAR macrophages directs them towards a pro-inflammatory phenotype and enhances their anti-tumor activities.
Anti-cancer immunotherapy approaches are increasingly coveted. Chimeric antigen receptor (CAR)-T cell therapy has been shown to be an effective treatment for hematological tumors, but the treatment of solid tumors still lacks effectiveness, due to lower intra-tumor infiltration of CAR-T cells and tu...
ORGANISM(S): Homo sapiens (Human) 
2024-12-12 | PXD058491 | Pride
Proprotein convertases Furin Breast cancer Car macrophage Tumoroids 
ProteomicsLocation of Neonatal Microglia Drives Small Extracellular Vesicles Content and Biological Functions
Combining proteomics and systems biology analyses, we demonstrated that neonatal microglial cells derived from two different CNS locations (cortex and spinal cord) displayed different phenotypes upon different physiological or pathological conditions. These cells also exhibited great variability in ...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-05-26 | PXD016093 | Pride
Exosomes Proteomics Lc-ms Microglia Vesicles 
ProteomicsIntegrated Mass Spectrometry Imaging and Omics Workflows on the Same Tissue Section Using Parafilm-Assisted Microdissection
In this work, we demonstrate the use of grid-aided, parafilm-assisted microdissection to perform MALDI MS imaging and shotgun proteomics and metabolomics in a combined workflow and using only a single tissue section. The grid is generated by microspotting of acid dye 25 using a piezoelectric microsp...
ORGANISM(S): Rattus norvegicus (Rat) 
2017-03-20 | PXD005345 | Pride
Proteomics Lipidomics Maldi ms imaging Parafilm-assisted microdissection 
ProteomicsHistone proteomic associated with epigenetic studies revealed a Ghost proteome in PC1/3 KD macrophages involved in anti - tumoral resistance under IL - 10 stimulation
In our previous work, our investigation on macrophages has allowed us to show that the inhibition of the enzyme proprotein convertase (PC1/3) controls the activation of macrophages. We demonstrated that PC1/3 knockdown (KD) in macrophages would exhibit an increased secretion of pro-inflammatory and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-02-12 | PXD020610 | Pride
Macrophage Lc-msms Altprots Histone Epigenetic 
ProteomicsEstablishment and characterization of a tumoroid biobank derived from dog patients’ mammary tumors for translational research
Breast cancer is the most frequent cancer among women causing the greatest number of cancer-related deaths. Cancer heterogeneity is a main obstacle to therapies. Around 96% of the drugs fail from discovery to the clinical trial phase probably because of the current unreliable preclinical models. New...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2023-03-11 | PXD031440 | Pride
Dog patients Drug screening Breast cancer Tumoroid Biobank 
ProteomicsHeterogeneity Assessment and Protein Pathway Prediction via Spatial Lipidomic and Proteomic Correlation: Advancing Dry Proteomics concept for Human Glioblastoma Prognosis
Prediction of proteins and associated biological pathways from lipid analyses via MALDI MSI is a pressing chal-lenge. We introduced "dry proteomics," using MALDI MSI to validate spatial localization of identified optimal clusters in lipid or protein imaging. Consistent cluster appearance across omic...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-05-07 | PXD054488 | Pride
Spatial proteomics Heterogeneity Dry proteomics Machine learn-ing Glioblastoma prognosis. Cluster estimation Maldi msi multi-omics 
ProteomicsQuick steps to get glioma extracellular vesicles surface membrane proteins mapping based on tryspin/Lysin C digestion followed by Amicon and shot gun proteomic: identification of ghost protein
Extracellular vesicles (EVs) are known to mediate intercellular communication and regulate a diverse range of crucial biological processes. Novel therapeutic strategies have emerged based on these EVs as biological nanoparticles. EVs is as a generic term for all secreted vesicles released including ...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-02-12 | PXD016944 | Pride
Glioma Vesicle Ghost-protein Lc-msms 
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