Sort   by:  
 Page size 
ProteomicsPhosphorylation of Cohesin's Deposition Factor, Scc2 Regulates its Deposition Activity
Phosphorylated residues of the budding yeast Scc2 protein were identified using phosphopeptide enrichment and mass spectrometry.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-09-10 | PXD001830 | Pride
Nipbl Scc2 Delangin Cohesin Sister chromatid cohesion 
ProteomicsLC/MS Analysis of Myc-Affinity Captured, on-Beads Crosslinked, and on-Beads Digested S. cerevisiae SCC2 subunit of cohesin loading factor
Myc-Affinity Capture and on-Beads Crosslinking For each Saccharomyces cerevisiae strain (C-terminally myc-tagged Scc2 subunit of the cohesin loading factor complex or wild-type BY4741), 2x 3 L YPD medium (add 300 ml 20% Glucose to each 3 L of YPD before using) were separately inoculated in a 5L flas...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-10-20 | MSV000086339 | MassIVE
GenomicsThe Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [ChIP-seq]
The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex ChIp- Sequencing analysis of the distribution of Scc2 and Scc4 on budding yeast chromosomes.An input sample ...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-01 | E-GEOD-56993 | biostudies-arrayexpress
GenomicsThe Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [Mnase-seq]
The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex MNase digestion of purified DNA followed by paired-end sequencing was performed in order to investigate geno...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-01 | E-GEOD-56992 | biostudies-arrayexpress
ProteomicsStructure of the Cohesin Loader Scc2-Scc4 and its Binding to a Folded Cohesin
The functions of cohesin are central to genome integrity, chromosome organization, and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex, however, little is k...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-01-11 | PXD004692 | Pride
Cohesin Cross-linking Cx-ms 
GenomicsGenowide-wide analysis of scc2-4 binding.
The evolutionarily conserved cohesin complex is crucial for holding sister chromatids together from the time of DNA replication until their segregation during the metaphase to anaphase transition. Human diseases associated with with mutations in the cohesin network are termed "cohesinopathies&q...
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2015-06-16 | E-GEOD-55357 | biostudies-arrayexpress
TranscriptomicsThe cohesin loader Scc2 regulates key regulatory genes critical for development
The evolutionarily conserved cohesin complex is crucial for holding sister chromatids together from the time of DNA replication until their segregation during the metaphase to anaphase transition. Human diseases associated with with mutations in the cohesin network are termed "cohesinopathies&q...
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2015-06-16 | E-GEOD-55316 | biostudies-arrayexpress
TranscriptomicsDisplacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres, where it provides the cohesive counter-force to bi-polar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences tran...
ORGANISM(S): Saccharomyces cerevisiae 
2007-09-29 | E-GEOD-8661 | biostudies-arrayexpress
GenomicsRNA Seq analysis of SCC2 mutations R787G and G1242V at 9 hours following SCC2 depletion in Saccharomyces cerevisiae
RNA Seq analysis of SCC2 mutations R787G and G1242V at 9 hours following SCC2 depletion in Saccharomyces cerevisiae
PRJNA828622 | ENA
GenomicsRNA Seq analysis of SCC2 mutations R787G and G1242V at 3 hours following SCC2 depletion in Saccharomyces cerevisiae
RNA Seq analysis of SCC2 mutations R787G and G1242V at 3 hours following SCC2 depletion in Saccharomyces cerevisiae
PRJNA828620 | ENA
Sort   by:  
 Page size