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ProteomicsThrombin activation of the factor XI dimer is a multi-staged process for each subunit
Factor XI (FXI) is a protein in the intrinsic coagulation pathway that can be activated by two enzymes. In hemostasis, FXI is activated by thrombin, while FXIIa-mediated activation of FXI is thought to be prothrombotic. The interactions of these enzymes with FXI are poorly understood due to their tr...
ORGANISM(S): Homo sapiens (Human) 
2024-02-05 | PXD039489 | Pride
Molecular dynamics Factor xi Crosslinking mass spectrometry Phox Thrombin 
ProteomicsPhoX - An IMAC-enrichable Crosslinking Reagent
Chemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry. However, sub-s...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Rattus norvegicus (Rat) 
2019-09-13 | PXD011931 | Pride
Phosphonic acid Xlinkx Tri-functional crosslinker Structural biology Crosslinking mass spectrometry Imac enrichable crosslinker 
ProteomicsQuantifying Positional Isomers (QPI) by top-down mass spectrometry
Proteomics has exposed a plethora of post-translational modifications, but demonstrating functional relevance requires new approaches. Top-down proteomics can characterize co-occurring modifications in terms of localization, abundance and hierarchy. Here, we present a top-down MS analysis workflow f...
ORGANISM(S): Homo sapiens (Human) 
2021-03-18 | PXD012084 | Pride
Top-down mass spectrometry Positional isomers Quantitation Phosphorylation 
ProteomicsMolecular snapshots of translation and protein translocation at the ER membrane
The dynamic ribosome-translocon complex, which resides at the endoplasmic reticulum (ER) membrane, produces a major fraction of the human proteome . It governs the synthesis, translocation, membrane insertion, N-glycosylation, folding and disulfide-bond formation of nascent proteins. While individua...
ORGANISM(S): Homo sapiens (Human) 
2023-02-03 | PXD035475 | Pride
Ribosome Er membrane Elongation factor 1 
ProteomicsType III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages
Characteristic for type III CRISPR-Cas systems is the generation of cyclic oligoadenylate (cOA) molecules, which allosterically activate proteins carrying cognate sensory domains: CARF or SAVED. Here, we characterize a type III-B system associated set of genes from Haliangium ochraceum, containing t...
ORGANISM(S): Haliangium ochraceum DSM 14365 
2025-05-06 | PXD046897 | Pride
Type iii crispr-cas 
ProteomicscapsPASEF – Collisional Cross Section Assisted Precursor Selection Applied to Cross-linking Mass Spectrometry
Ion mobility separates molecules in the gas-phase on their physico-chemical properties, providing information about their size as collisional cross-sections. The timsTOF Pro combines trapped ion mobility with a quadrupole, HCD cell and a time-of-flight mass spectrometer, to interrogate ions at high ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-07-22 | PXD018189 | Pride
Ion mobility Cross-linking mass spectrometry Xl-ms Phox 
ProteomicsInceptor binds and directs insulin to lysosomal degradation in -cells
Blunted first-phase insulin secretion and insulin deficiency are early signs of -cell dysfunction and diabetes manifestation. Thus, insights into molecular mechanisms that regulate insulin homeostasis might provide entry sites to replenish insulin content and restore -cell function. Here, we identif...
ORGANISM(S): Homo sapiens (Human) 
2024-09-09 | PXD040758 | Pride
Cdms Inceptor 
ProteomicsThe Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High Performance Quadrupole and an Ultra-High Field Orbitrap Analyzer
The quadrupole Orbitrap mass spectrometer (Q Exactive) has made a powerful proteomics instrument available in a benchtop format. It significantly boosted analyzable proteins per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus ...
ORGANISM(S): Homo sapiens (Human) 
2014-11-10 | PXD001203 | Pride
Segmented quadrupole Q exactive hf Orbitrap ultra-high field Q exactive plus Quadrupole pre-filter Shotgun proteomics 
ProteomicsLife history shapes variation in egg composition in a wild passerine
Maternal investment directly shapes early developmental conditions and therefore has long-term fitness consequences for the offspring. In oviparous species prenatal maternal investment is fixed at the time of laying. To ensure the best survival chances for most of their offspring, females must equi...
ORGANISM(S): Cyanistes caeruleus 
2019-01-07 | PXD009822 | Pride
Differential allocation Cyanistes caeruleus 
ProteomicsThe Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High Performance Quadrupole and an Ultra-High Field Orbitrap Analyzer
The quadrupole Orbitrap mass spectrometer (Q Exactive) has made a powerful proteomics instrument available in a benchtop format. It significantly boosted analyzable proteins per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-04-01 | MSV000080865 | MassIVE
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