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ProteomicsComprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases
We analyzed total proteome of three methyltransferase gene knockouts strains of Escherichia coli: ΔrsmF (JW5301), ΔrlmC (JW2756), ΔrlmE (JW3146) from Keio collection and wild type (WT). Proteins were assessed using IDA approach (i.e. Information Dependent Acquisition) and SWATH (Data-Independent Acq...
ORGANISM(S): Escherichia coli 
2020-01-21 | PXD017171 | Pride
Lc-msms Escherichia coli Ida 
ProteomicsProteome of wild type and Nsun7 knockout mice elongated spermatids
Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal column...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-12 | PXD067394 | Pride
Nsun7 Elongated spermatids Spermatogenesis Longitudinal columns Methyltransferase 
ProteomicsProteome of wild type and Nsun7 knockout mice round spermatids
Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal column...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-12 | PXD067398 | Pride
Nsun7 Spermatogenesis Round spermatids Longitudinal columns Methyltransferase 
ProteomicsProteome of wild type and Nsun7 knockout mice spermatozoa
Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal column...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-12 | PXD067410 | Pride
Spermatozoa Nsun7 Spermatogenesis Longitudinal columns Methyltransferase 
ProteomicsProteome of wild type and Nsun7 knockout mice testis
Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal column...
ORGANISM(S): Mus musculus (Mouse) 
2025-03-07 | PXD057007 | Pride
Testis Nsun7 Spermatogenesis Longitudinal columns Methyltransferase 
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