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ProteomicsGlobal quantitative analysis of the human brain proteome in Alzheimer’s and Parkinson’s Disease
Patients with Alzheimer’s disease (AD) and Parkinson disease (PD) often have overlap in brain neuropathology and clinical presentation suggesting that these two diseases share common underlying mechanisms. Currently, the molecular events linking AD and PD are incompletely understood. Utilizing 10-p...
ORGANISM(S): Homo sapiens (Human) 
2018-02-07 | PXD007160 | Pride
Brain Tmt Human Sps Parkinson's Alzheimer's Lc-ms/ms 
ProteomicsComparison of microglial proteomes of MACS and FACS-based purified CD11b+ microglia
Background Proteomic characterization of microglia has been limited by low yield and contamination by non-microglial proteins by magnetic-activated cell sorting (MACS) enrichment strategies. To determine whether a fluorescent-activated cell sorting (FACS)-based strategy overcomes these limitations,...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-11 | PXD015652 | Pride
Mouse acutely purified brain-associated microglia Tmt Macs Facs 
ProteomicsA U1-70K Protein Interaction Network Reveals a Functional Class of RNA Binding Proteins with Mixed Charge Domains
U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) and other proteins of the spliceosome complex are mislocalized to cytoplasmic aggregates in Alzheimer’s disease (AD) brain, yet understanding of the specific mechanisms that cause their aggregation is limited. Many of the RNA binding proteins (RBPs)...
ORGANISM(S): Homo sapiens (Human) 
2018-06-21 | PXD008260 | Pride
U1-70k 
ProteomicsAllele-Specific Protein Abundance in Human Brain
We describe a method for identifying peptides that result from missense changes and identify peptides among 2 human brains that would have otherwise not been detected. Next, we use this data to estimate of allele-specific protein abundance in human brain for an average per individual, and to estimat...
ORGANISM(S): Homo sapiens (Human) 
2017-07-31 | PXD004143 | Pride
Proteogenomics genomics variants proteomics 
ProteomicsPosttranslational modifications mediate the structural diversity of tauopathy strains
Tau aggregation is the defining pathological hallmark of tauopathies, but structural insights are revealing key differences between filaments across this class of disorders. Using cryo-electron microscopy, we identify a 107-residue fragment, K274-E380, which forms the insoluble core of tau filaments...
ORGANISM(S): Homo sapiens (Human) 
2020-02-14 | PXD016862 | Pride
Cbd Tau Mass spectrometry 
ProteomicsMembrane-Enriched Brain Proteome Across Stages of Alzheimer's Disease
Previous systems-based proteomic approaches have characterized alterations in protein co-expression networks of unfractionated asymptomatic (AsymAD) and symptomatic Alzheimer’s disease (AD) brains. However, it remains unclear how sample fractionation and sub-proteomic analysis influences the organi...
ORGANISM(S): Homo sapiens (Human) 
2019-09-02 | PXD014376 | Pride
Ad Synaptic proteome Transmembrane proteins 
ProteomicsMiddle-down proteomics reveals RNA binding proteins harbor dense sites of methylation and phosphorylation
Arginine (Arg)-rich RNA-binding proteins play an integral role in RNA metabolism. Post-translational modifications (PTMs) within Arg-rich domains, such as phosphorylation and methylation, regulate multiple steps in RNA metabolism. However, the identification of PTMs within Arg-rich domains with comp...
ORGANISM(S): Homo sapiens (Human) 
2020-01-31 | PXD015208 | Pride
Middle-down Rs protein Etd Decision tree Basic-acidic domains 
ProteomicsAlzheimer Disease (AD) and AD model detergent insoluble proteomes
Characterizing the detergent insoluble brain proteome of sporadic late-onset Alzheimer’s disease (LOAD) has identified proteins and pathways associated with disease pathogenesis. Similar studies in early onset Alzheimer’s disease cases due to presenilin-1 mutations (PS1-EOAD), along with more detail...
ORGANISM(S): Homo sapiens (Human) 
2019-06-25 | PXD005321 | Pride
In-gel digestion Dquan Neurodegeneration In house xic quantitation 
ProteomicsQuantitative Proteomics of Acutely Isolated Mouse Microglia Identifies Novel Immune Alzheimer's Disease-related Proteins
Microglia are innate immune cells of the brain that perform phagocytic and inflammatory functions in disease conditions. Transcriptomic studies of acutely-isolated microglia have provided novel insights into their molecular and functional diversity in homeostatic and neurodegenerative disease states...
ORGANISM(S): Mus musculus (Mouse) 
2018-09-18 | PXD009137 | Pride
Cd11b-positive Brain resident microglia 
ProteomicsTargeted quantification of detergent-insoluble RNA-binding proteins in Alzheimer’s disease reveals disease-specific coaggregation signatures
Core spliceosome and related RNA-binding proteins aggregate in Alzheimer’s disease (AD) brain even in early asymptomatic stages (AsymAD) of disease. To assess the specificity of RNA-binding protein aggregation in AD, we developed a targeted mass spectrometry approach to quantify broad classes of RNA...
ORGANISM(S): Homo sapiens (Human) 
2021-03-17 | PXD022144 | Pride
Parkinson’s disease Alzheimer’s disease Insoluble proteins Rna-binding proteins Parallel reaction monitoring (prm) mass spectrometry Human brains 
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