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ProteomicsQuantitative proteomic analysis of Shigella flexneri and Shigella sonnei Generalized Modules for Membrane Antigens (GMMA) reveals highly pure preparations.
Shigella flexneri 2a and Shigella sonnei were genetically modified to shed large quantities of outer membrane blebs. The blebs, called Generalized Modules for Membrane Antigens (GMMA), were purified and the protein content was estimated using the label-free iBAQ procedure. There were 2308 proteins i...
ORGANISM(S): Shigella flexneri 2a str. 2457T Shigella sonnei 53G 
2016-01-12 | PXD002517 | Pride
Shigella flexneri Label-free quantification Shigella sonnei. lc-ms/ms 
TranscriptomicsExpression analysis of Shigella flexneri 2a strain 301
Investigation of whole genome gene expression to identify overlooked sRNAs and sORFs. Background The completion of numerous genome sequences has introduced an era of whole-genome study. However, many real genes, including small RNAs (sRNAs) and small ORFs (sORFs), are missed in genome annotation. In...
ORGANISM(S): Shigella flexneri 2a str. 301 
2011-06-27 | E-GEOD-22800 | biostudies-arrayexpress
TranscriptomicsDual-transcriptome of Shigella flexneri infecting human HCT116 cells
Analyse the transcriptionnal changes of human colon epithelial cells (HCT116) associated with Shigella flexneri (M90T) infection and correlated with the bacterial transcriptional program.
ORGANISM(S): Homo sapiens 
2024-12-01 | E-MTAB-14119 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of newborn mice infected with Shigella flexneri
In order to identify the developmental changes controlling the switch from disease susceptibility to resistance, we performed global gene expression analysis on non-infected and infected intestinal tissues taken from 4-day- and 7-day-old animals. Experiment Overall Design: RNA from intestinal tissue...
ORGANISM(S): Mus musculus 
2008-04-04 | E-GEOD-9785 | biostudies-arrayexpress
ProteomicsAPEX2-based mapping of the host-Shigella interface
To discover novel factors that modulate S. flexneri infection, we developed a proteomic-based approach based on the biotin ligation enzyme APEX2. To target the host-Shigella interface, we coated bacteria in vitro prior to infection using anti-GFP nanobody-expressing shigella and recombinant GFP-APE...
ORGANISM(S): Shigella flexneri Homo sapiens (Human) Shigella 
2026-08-17 | PXD063609 | Pride
Shigella Apex2 Human Biotin 
ProteomicsProtein identification of BS3 crosslinked Shigella flexneri type III secretion system needle complex
Protein identification of BS3 crosslinked Shigella flexneri type III secretion system needle complex
ORGANISM(S): Shigella flexneri 5a 
2026-05-05 | PXD038227 | Pride
Shigella flexneri Type iii secretion system needle complex 
ProteomicsLC-MS/MS of the secreted fraction of Shigella
Many human Gram-negative bacterial pathogens express a Type Three Secretion Apparatus (T3SA), including among the most notorious Shigella spp., Salmonella enterica, Yersinia enterocolitica and enteropathogenic Escherichia coli (EPEC). These bacteria express on their surface multiple copies of the T3...
ORGANISM(S): Shigella flexneri 
2017-10-17 | PXD006086 | Pride
Shigella Plasmid Gram-negative bacterial pathogens Proteomics 
ProteomicsEscherichia coli killing by Shigella sonnei is mediated by colicins found in epidemiologically successful sublineages
Shigella sonnei as an enteric pathogen, can benefit from outcompeting gut commensals such as Escherichia coli and there can be several mechanisms that assist in interbacterial competition. It is, therefore, important to understand these mechanisms as they provide insights into the interactions of th...
ORGANISM(S): Shigella sonnei 
2023-10-24 | PXD036656 | Pride
Colicins Bper Shigella 
ProteomicsEffects of gene knockout on Shigella proteome
We measured the impact of multiple genes on the proteome of Shigella.
ORGANISM(S): Shigella flexneri 5b 
2025-03-07 | PXD046629 | Pride
Knockout Shigella 
TranscriptomicsComparative Genomic Hybridization of E. coli and Shigella species to determine the relatedness of isolates from Neobladders
Using comparative genomic hybridization we examined the genome content of 30 isolates of E. coli and Shigella to determine the relative location of E. coli isolates from the human neobladder Isolates were included in the study that represent the prototype strains of multiple pathovars. No replicate...
ORGANISM(S): Shigella sonnei 
2011-07-24 | E-GEOD-27326 | biostudies-arrayexpress
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