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ProteomicsReproducibility of label-free single-shot phosphoproteomics applied to CRC cell lines
There is a need for robust phosphopeptide enrichment methods to allow signaling network analysis in cancer cell lines and tissues with minimal fractionation. With recent instrument developments thousands of unique phosphopeptides can be detected by single-shot LC-MS/MS. However, successful phosphopr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080715 | MassIVE
ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
ProteomicsMicro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) 
2021-07-01 | PXD023587 | Pride
Single shot proteomics Micro-flow lc 1.0 mm id column 
ProteomicsMulti-omic Single-Shot Technology for Integrated Proteome and Lipidome Analysis
This is the MOST raw data files including 20 biological conditions in triplicate.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606) 
2020-07-27 | MSV000085838 | MassIVE
ProteomicsDeep proteome analysis by DIA-MS with FAIMS
We aimed to expand the proteome coverage by single-shot measurements using optimizing high-field asymmetric waveform ion mobility spectrometry (FAIMS) parameters in DIA-MS. In addition, we analyzed host proteins in the feces of control and repeated social defeat stress (R-SDS) model mice with optima...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
2022-04-28 | PXD029853 | JPOST Repository
ProteomicsSingle-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms
Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) has been recognized as an invaluable platform for top-down proteomics. However, the scale of top-down proteomics from CZE-MS/MS is still limited due to the low loading capacity and narrow separation windo...
ORGANISM(S): Escherichia coli 
2017-10-30 | PXD007273 | Pride
Top-down proteomics Cze-ms/ms E.coli 
ProteomicsReproducibility of label-free single-shot phosphoproteomics applied to CRC cell lines
There is a need for robust phosphopeptide enrichment methods to allow signaling network analysis in cancer cell lines and tissues with minimal fractionation. With recent instrument developments thousands of unique phosphopeptides can be detected by single-shot LC-MS/MS. However, successful phosphopr...
ORGANISM(S): Homo sapiens (Human) 
2015-04-15 | PXD001546 | Pride
Human Single-shot Crc Label-free Phosphoproteomics Colorectal cancer 
UnknownComprehensive Single-Shot Proteomics with FAIMS on a Hybrid Orbitrap Mass Spectrometer.
Not available
S-EPMC6145172 | biostudies-literature
UnknownMapping the Melanoma Plasma Proteome (MPP) Using Single-Shot Proteomics Interfaced with the WiMT Database.
Not available
S-EPMC8699267 | biostudies-literature
ProteomicsMapping the Melanoma Plasma Proteome (MPP) using Single-Shot Proteomics and Custom Protein Databases
We mapped the Melanoma Plasma Proteome (MPP) of undepleted samples a new strategy for protein identification refered here as Wise MS transfer (WiMT). We were able to identify more than 1,000 protein in undepleted plasma samples which shows a deep coverage of the plasma proteome. The MPP of undeplted...
ORGANISM(S): Homo sapiens (Human) 
2021-12-27 | PXD028709 | Pride
Malignant melanoma  plasma  proteome  proteomics  biomarkers  wimt 
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