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ProteomicsThe Importance of Extracellular Vesicle Purification for Downstream Analysis: A Comparison of Differential Centrifugation and Size Exclusion Chromatography for Parasitic Helminths
Fascioliasis is a major neglected tropical disease that infects humans and ruminant species worldwide. Recent discoveries have identified that liver fluke extracellular vesicles are released into the host’s internal environment. To understand extracellular vesicle biology further, isolation of the e...
ORGANISM(S): Fasciola hepatica (Liver fluke) 
2019-02-13 | PXD008737 | Pride
Extracellular vesicles Size exclusion chromatography and fasciola hepatica. Purification Differential centrifugation 
ProteomicsGlobal profiling of the RNA and protein complexes of Escherichia coli by size exclusion chromatography followed by RNA sequencing and mass spectrometry (SEC-seq)
21 total proteome samples from E. coli MG1655 wild type fractionated by size exclusion chromatography resulting in 20 fractions. A lysate sample is given as input control.
ORGANISM(S): Escherichia coli 
2022-11-15 | PXD036475 | Pride
Size exclusion chromatography E. coli Lc-ms/ms 
ProteomicsSeparation of proteome of Kuenenia stuttgartiensis strain CSTR1 using size exclusion chromatography in 0.5 mL fractions
We analyzed proteome of the anammox bacterium Kuenenia stuttgartiensis strain CSTR1 after separation by size exclusion chromatography. Each fraction has a volume of 0.5 mL.
ORGANISM(S): Candidatus Kuenenia stuttgartiensis 
2025-02-03 | PXD054988 | Pride
Size exclusion chromatography Anammox Proteome 
ProteomicsSeparation of proteome of Kuenenia stuttgartiensis strain CSTR1 using size exclusion chromatography in 0.1 mL fractions
We analyzed proteome of the anammox bacterium Kuenenia stuttgartiensis strain CSTR1 after separation by size exclusion chromatography. Each fraction has a volume of 0.1 mL. Elution volume is between 11.5 and 13.9 mL
ORGANISM(S): Candidatus Kuenenia stuttgartiensis 
2025-02-03 | PXD054995 | Pride
High resolution fractionation Size exclusion chromatography Anammox Proteome 
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
Proteomicsdichotomic size-exclusion chromatography（SEC）
directDIA proteomics raw data of plasma extracellular vesicles isolated by SEC and UC
ORGANISM(S): Homo Sapiens 
2021-05-17 | PXD026034 |
ProteomicsTop-down Proteomics of Large Proteins Up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy
Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and post-translational modifications (PTMs). However, top-down MS analysis of high molecular weight (MW) proteins remains challenging mainly due to t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-11-29 | MSV000080365 | MassIVE
ProteomicsSelf-packed size-exclusion columns enable versatile high-throughput native, top-down, and ion-mobility-mass spectrometry studies on proteins and complexes.
Native MS (nMS) is a key structural biology technique that makes it possible to study intact proteins and their interactions. Unfortunately, non-volatile salts are incompatiblewith nMS, which demands a laborious desalting procedure. Non-denaturing size exclusion chromatography (SEC) allows both rapi...
ORGANISM(S): Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2025-06-29 | PXD061452 | Pride
Ligand screening Top-down proteomics Native mass spectrometry Size-exclusion chromatography Protac Ion mobility 
ProteomicsFractionation of Naja ashei venom with Size-exclusion chromatography and identification of proteins in obtained fractions
The study aimed to fractionate Naja ashei venom with Size-exclusion chromatography to obtain fractions with significantly lower complexity than crude venom. MS analysis allowed to estimate the percentage of different protein groups in each fraction, thus providing information about the extent of hom...
ORGANISM(S): Naja ashei 
2021-09-10 | PXD023801 | Pride
Shotgun lc-ms/ms Venom Naja ashei Size-exclusion chromatography 
ProteomicsSize-exclusion chromatography of soluble chloroplast proteins from Nicotiana tabacum (tobacco)
Maturase K (MatK) is the only splicing factor encoded in the chloroplast genomes of land plants. Here we used a transplastomic Nicotiana tabacum line, in which the plastidial MatK gene is C-terminally tagged with a 3x-HA tag, to conduct size-exclusion chromatography of soluble protein extracts enric...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD069946 | Pride
Maturase k Lc-ms/ms Tobacco Dia Size-exclusion chromatography Chloroplast 
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