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ProteomicsSupercharging-Enhanced nDIA-MS Enables Global Profiling of Drug-Induced Proteome Solubility Shifts (solubility proteomics)
Mass spectrometry (MS)-based proteomics has become indispensable for high-throughput quantitation of protein expression. However, protein function is regulated by a variety of factors beyond abundance alone. Here, we optimized narrow-window, data-independent ac-quisition (nDIA) MS with 3% DMSO as a ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD064185 | Pride
Ml-792 Proteome Solubility shift Mg132 
ProteomicsSelection of Heating Temperatures Improves the Sensitivity of Proteome Integral Solubility Alteration (PISA) Assay
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD017346 | Pride
Proteome integral solubility alteration (pisa) assay Human cell line lysate Lc-ms/ms 
ProteomicsNeurodegenerative disease mouse model SDS-insoluble proteins
The deposition of pathologic misfolded proteins in neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, frontotemporal dementia and amyotrophic lateral sclerosis is hypothesized to burden protein homeostatic (proteostatic) machinery, potentially leading to insufficient capac...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-29 | PXD010828 | Pride
Protein misfolding Transgenic mice Proteomics Lc-ms/ms 
ProteomicsProteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways
To simplify chemical risk assessment, the adverse outcome pathway (AOP) method has arisen as a framework to predict the impact of chemical exposure on human and environmental health. The development of this predictive tool requires knowledge of the molecular interaction between chemicals and protein...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD033056 | Pride
Hepatocytes Bioactive compound Pollutants Proteome integral solubility alteration assay Tcdd Analytical hierarchy process Hepg2 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
UnknownThe Total Solubility of the Co-Solubilized PAHs with Similar Structures Indicated by NMR Chemical Shift.
Not available
S-EPMC8125976 | biostudies-literature
ProteomicsPhosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization
Post-translational modifications (PTMs) within splicing factor RNA-binding proteins (RBPs), such as phosphorylation, regulate several critical steps in RNA metabolism including spliceosome assembly, alternative splicing and mRNA export. Notably, the arginine-/serine-rich (RS) domains in SR proteins ...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD026894 | Pride
Post-translational modifications Protein interactions Srsf2 Mass spectrometry Phosphorylation Rna-binding proteins Sc35 
ProteomicsAssessing target engagment using proteome-wide solvent shift assays
Recent advances in mass spectrometry (MS) have enabled quantitative proteomics to become a powerful tool in the field of drug discovery, especially when applied toward proteome-wide target engagement studies. Similar to temperature gradients, increasing concentrations of organic solvents stimulate u...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026297 | Pride
Target enagagement 
ProteomicsDrPISA: Deep Eutectic Solvent-Assisted Reverse Proteome-Integrated Solubility Alteration for High-Sensitivity Drug Target Identification
Existing thermal shift-based mass spectrometry approaches, such as TPP and PISA, focus mainly on soluble fractions, often neglecting the insoluble pellet, limiting comprehensive drug–target profiling. In this work, we developed DrPISA (deep eutectic solvent-assisted reverse PISA), a novel strategy t...
ORGANISM(S): Homo Sapiens 
PXD066145 |
UnknownThermodynamic Correlation between Liquid-Liquid Phase Separation and Crystalline Solubility of Drug-Like Molecules.
Not available
S-EPMC9782016 | biostudies-literature
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