Sort   by:  
 Page size 
ProteomicsSpatial SILAC Regorafenib Treated Bottom-up Proteomic Analysis
Developing effective model systems to evaluate new potential chemotherapeutic reagents is critical. Three-dimensional cell cultures, such as spheroids, aid in bridging the gap between commonly used monolayer cell cultures and significantly more complex and expensive animal models. Spheroid model sys...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD038958 | Pride
Spatial silac Regorafenib Spheroids 
ProteomicsMapping the spatial proteome of metastatic cells in colorectal cancer
Here, we aimed to study metastasis mechanisms using spatial proteomics applied to the KM12 cell model of metastasis. KM12 cells were metabolically labelled using SILAC. SILAC has been successfully used for the analysis of proteome turnover and for the identification of changes in proteome localizati...
ORGANISM(S): Homo sapiens (Human) 
2017-09-11 | PXD006656 | Pride
Subcellular fraction Colorectal cancer Silac Lc-ms/ms 
ProteomicsMapping the spatial proteome of metastatic cells in colorectal cancer
Here, we aimed to study metastasis mechanisms using spatial proteomics applied to the KM12 cell model of metastasis. KM12 cells were metabolically labelled using SILAC. SILAC has been successfully used for the analysis of proteome turnover and for the identification of changes in proteome localizati...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-11-23 | MSV000088431 | MassIVE
ProteomicsProteomic analysis of nascent proteins in proximity to DFCP1 using SILAC labeling and APEX2-mediated biotin-streptavidin affinity purification.
This study employs a spatial proteomics approach combining APEX2 proximity labeling with SILAC (Stable Isotope Labeling by Amino acids in Cell culture) to map the nascent proteome in the vicinity of DFCP1.
ORGANISM(S): Homo sapiens (Human) 
2026-02-21 | PXD073558 | Pride
Proximity labeling Autophagy Apex2 Silac Nascent proteins Dfcp1 
ProteomicsA quantitative spatial proteomics analysis of proteome turnover in human cells
Measuring the properties of endogenous cell proteins, such as expression level, subcellular localization and turnover rates, on a whole proteome level remains a major challenge in the post-genome era. Quantitative methods for measuring mRNA expression do not reliably predict corresponding protein le...
ORGANISM(S): Homo sapiens (Human) 
2015-12-01 | PXD003258 | Pride
Hela Subcellular Silac 
ProteomicsProteomic Sensors for Quantitative, Multiplexed, and Spatial Monitoring of Kinase Signaling
Proteomic Kinase Activity Sensors (ProKAS) is a new technology developed to allow for quantitative and multiplexed analyses of kinase signaling in living cells with spatial resolution. Kinase sensors are expressed in cells as part of a ProKAS construct, which uses signal peptides to localize to spec...
ORGANISM(S): Homo sapiens (Human) 
2025-10-02 | PXD065795 | Pride
Proteomics atr atm chk1 dna damage genome maintenance signaling phosphoproteomics prm 
UnknownSpatial Stable Isotopic Labeling by Amino Acids in Cell Culture: Pulse-Chase Labeling of Three-Dimensional Multicellular Spheroids for Global Proteome Analysis.
Not available
S-EPMC9248019 | biostudies-literature
ProteomicsAP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A
Adaptor protein 4 (AP-4) is an ancient membrane trafficking complex, whose function has largely remained elusive. In humans, AP-4 deficiency causes a severe neurological disorder of unknown aetiology. We apply unbiased proteomic methods, including ‘Dynamic Organellar Maps’, to find proteins whose su...
ORGANISM(S): Homo sapiens (Human) 
2018-09-27 | PXD010103 | Pride
Human cells Autophagy Dynamic organellar maps Ap-4 Vesicles 
ProteomicsDefinition and characterization of the mitochondrial proteome of human cells (MitoCoP)
Aim of this project was the definition and characterization of the mitochondrial proteome of human cells, referred to as MitoCoP. To this end, we combined the complementary strengths of subtractive proteomics, ImportOmics and subcellular protein profiling in a global multidimensional classification ...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD016924 | Pride
Proteomics Silac Subtractive proteomics Mitochondria Spatial proteomics Pulsed silac Importomics Complexome profiling Rnai-mediated protein knockdown 
ProteomicsGlobal Subcellular Characterization of Protein Degradation Using Quantitative Proteomics
Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass ...
ORGANISM(S): Homo sapiens (Human) 
2015-11-27 | PXD003239 | Pride
Cytosolic U2os Cytoskeletal Prr11 Osteosarcoma cells Nuclear Membrane 
Sort   by:  
 Page size