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ProteomicsProteomic analysis of human monosomic cell lines, Label Free MS
Chromosome loss that results in monosomy has detrimental consequences for human cells, but the underlying molecular mechanisms remain enigmatic. Using p53 deficient monosomic cell lines, we found that chromosome loss impairs proliferation and genomic stability. Transcriptome and proteome analysis re...
ORGANISM(S): Homo sapiens (Human) 
2021-06-17 | PXD022927 | Pride
Aneuploidy Lc-ms/ms Q exactive Label free quantification 
ProteomicsScaling of cellular processes with ploidy
While most eukaryotic cells are diploid, with two chromosome sets, variances in ploidy are common. Despite the relative prevalence of ploidy changes and their relevance for pathology and evolution, a complete picture of consequences of altered ploidy is missing. We analyzed transcriptome and proteom...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-15 | PXD022605 | Pride
Lc-msms Yeast Silac Ploidy 
ProteomicsProteomic analysis of human monosomic cell lines
Chromosome loss that results in monosomy has detrimental consequences for human cells, but the underlying molecular mechanisms remain enigmatic. Using p53 deficient monosomic cell lines, we found that chromosome loss impairs proliferation and genomic stability. Transcriptome and proteome analysis re...
ORGANISM(S): Homo sapiens (Human) 
2021-06-17 | PXD018440 | Pride
Tmt Monosomy Aneuploidy Lc-ms/ms 
ProteomicsThe ER protein Ema19 facilitates the degradation of non-imported mitochondrial precursor proteins
For the biogenesis of mitochondria, hundreds of proteins need to be targeted from the cytosol into the various compartments of this organelle. The intramitochondrial targeting routes these proteins take to reach their respective location in the organelle are well understood. However, the early targe...
ORGANISM(S): Chlamydomonas reinhardtii Saccharomyces cerevisiae (Baker's yeast) 
2021-03-24 | PXD022660 | Pride
Mitochondrial protein import Ae-ms Lc-ms/ms Mitochondria 
ProteomicsThe proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network
In plant cells, chloroplast gene expression is predominantly controlled through regulation of translation. Fine-tuning of protein synthesis is vital for plant development, acclimation to environmental challenges and for the assembly of major protein complexes such as the photosynthesis machinery. Ho...
ORGANISM(S): Chlamydomonas reinhardtii 
2021-01-04 | PXD020377 | Pride
Ribosome Chlamydomonas rheinhardtii Lcms Chloroplast 
ProteomicsThe proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network - Effect of Crosslinking
In plant cells, chloroplast gene expression is predominantly controlled through regulationof translation. Fine-tuning of protein synthesis is vital for plant development, acclimation to environmental challenges and for the assembly of major protein complexes such as the photosynthesis machinery. How...
ORGANISM(S): Chlamydomonas reinhardtii 
2020-11-23 | PXD022210 | Pride
Chlamydomonas Plastide ribosome Ae-ms Lc-ms/ms 
ProteomicsThe mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress
Most mitochondrial proteins are synthesized as precursor proteins in the cytosol and post-translationally transported into mitochondria. The mitochondrial surface protein Tom70 acts at the interface of the cytosol and mitochondria. In vitro import experiments identified Tom70 as targeting receptor, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-29 | PXD021173 | Pride
Chaperones  mitochondria  outer membrane  proteostasis  tom70 
ProteomicsProteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links part 2 of 2: AP-MS DATA (QUBIC)
DNA interstrand crosslinks (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. Here we establi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-13 | MSV000079577 | MassIVE
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080831 | MassIVE
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