Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs)....
We applied an optimized biotinylation approach for quantitative GeLC-MS based analysis of the staphylococcal cell-surface proteome and analyzed the cytoplasmic protein fraction as well in order to elucidate proteomic differences between colony biofilms and planktonic cells.
Cx32 wildtype and mutants (W3S, R22G) were expressed in HeLa cells and subjected to surface biotinylation to check for expression level differences between the different conditions. A CFP control was conducted.
Cx32 wildtype and mutants (W3S, R22G) were expressed in HEK293F cells and subjected to surface biotinylation to check for expression level differences between the different conditions. A CFP control was conducted.
In the past years several protocols for the proteomic profiling of plasma membrane proteins have been described. Nevertheless, comparative analyses have mainly focused on different variations of one approach [1-3]. To pave the way to high-performance differential plasma membrane proteomics, we compa...
Background: Cell surface proteins perform critical functions related to immune response, signal transduction, cell-cell interactions, and cell migration. Expression of specific cell surface proteins can determine cell-type identity, and can be altered in diseases including infections, cancer and gen...
