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Two-dimensional separation by nano-LC and trapped ion mobility spectrometry (TIMS) prior to Q-TOF tandem mass spectrometry significantly improves the accuracy of isobaric tag-based quantitation in proteome analysis without the need for additional measurement time for TIMS insertion. The obtained pea...
ORGANISM(S): Cellular Organisms 
Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-08-23 | MSV000084230 | MassIVE
Purpose: Increasing genomics-based evidence suggests that synchronous endometrial and ovarian cancer (SEOC) represents clonally related primary and metastatic tumors. A systematic analysis of the global protein landscape of SEOCs, heretofore lacking, could reveal functional and disease-specific cons...
ORGANISM(S): Homo sapiens (Human) 
2025-03-11 | PXD059172 | Pride
Anastrepha ludens (Loew) is one of the most important pestiferous fruit fly species infesting citrus and mangoes from Texas to Costa Rica. Despite the latter, the mechanisms of odorant perception in this specie have been poorly studied. In most of the cases, the detection of semiochemicals in inse...
ORGANISM(S): Anastrepha ludens 
2021-09-09 | PXD020012 | Pride
We present a new high field asymmetric waveform ion mobility spectrometry (FAIMS) interface that can be coupled to the Orbitrap Tribrid mass spectrometers. The interface provides several advantages over previous generations of FAIMS devices including ease of operation, robustness, and high ion trans...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-04-19 | MSV000082293 | MassIVE
Mechanistic study of H2S interference on swine, we used TMT-based comparative proteomics of lung tissues from control and hydrogen sulfide (H2S) breathing swines as a test case for evaluation of MS2, SPS MS3 and RTS-MS3 acquisition methods in both Orbitrap Fusion and Orbitrap Eclipse.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2023-06-02 | PXD020392 | Pride
The whole blood samples were obtained from 3 LTNPs and 2 RPs HIV-1 infected patients, and the CD4+ T cells were isolated using anti-CD4-specific antibody-coated microbeads (130-045-101, Miltenyi Biotec, Germany) according to the manufacturer's instructions. Then the proteins extracted from the CD4+ ...
ORGANISM(S): Homo Sapiens 
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Homo sapiens (Human) 
2020-05-15 | PXD018357 | Pride
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