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ProteomicsTargeted Protein Acetylation in Cells Using Heterobifunctional Molecules
Protein acetylation is a central event in orchestrating diverse cellular processes. However, current strategies to investigate protein acetylation in cells are often non-specific or lack temporal and magnitude control. Here, we developed an acetylation tagging system, AceTAG, to induce acetylation o...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD027617 | Pride
Targeted acetylation Acetylproteomics P300/cbp Heterobifunctional molecules 
MetabolomicsElucidation of xenoestrogen metabolism by non-targeted, stable isotope-assisted mass spectrometry in breast cancer cells

Environmental exposure to xenoestrogens, i.e., chemicals that imitate the hormone 17β-estradiol, has the potential to influence hormone homeostasis and action. Detailed knowledge of xenobiotic biotransformation processes in cell models is key when transferring knowledge learned from in vitro<...

2023-10-26 | MTBLS2716 | MetaboLights
GenomicsPromoter targeted histone acetylation of chromatinized parvoviral genome is essential for infrection progress
The association of host histones with parvoviral DNA is poorly understood. We analyzed the chromatinization and histone acetylation of canine parvovirus DNA during infection by confocal imaging and in situ proximity ligation assay combined with chromatin immunoprecipitation and high-throughput seque...
ORGANISM(S): Felis catus 
2016-02-11 | E-GEOD-77785 | biostudies-arrayexpress
ProteomicsAntibody-free targeted proteomics assay for absolute measurement of α-tubulin acetylation
Acetylation of α-tubulin at conserved lysine 40 (K40) amino acid residue regulates microtubule dynamics and controls a wide range of cellular activities. Dysregulated microtubule dynamics characterised by differential α-tubulin acetylation is a hallmark of cancer, neurodegeneration and other complex...
ORGANISM(S): Homo Sapiens Mus Musculus 
2020-07-30 | PXD018626 | panorama
GenomicsDUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes
Ectopic expression of the double homeodomain transcription factor DUX4 causes facioscapulohumeral muscular dystrophy (FSHD). Mechanisms of action of DUX4 are currently unknown. Using immortalized human myoblasts with a titratable DUX4 transgene, we identify by mass spectrometry an interaction betwee...
ORGANISM(S): Homo sapiens 
2016-03-10 | E-GEOD-78158 | biostudies-arrayexpress
ProteomicsAnalysis of acetylation stoichiometry suggests that SIRT3 repairs nonenzymatic acetylation lesions
Acetylation is frequently detected on mitochondrial enzymes and the sirtuin deacetylase SIRT3 is thought to regulate metabolism by deacetylating mitochondrial proteins. However, the stoichiometry of acetylation has not been studied and is important for understanding whether SIRT3 regulates or suppre...
ORGANISM(S): Mus musculus (Mouse) 
2015-09-15 | PXD000885 | Pride
Sirt3 Stoichiometry Acetylation Liver 
TranscriptomicsCfp1 is required for gene expression dependent H3K4me3 and H3K9 acetylation in embryonic stem cells (RNA-Seq)
Trimethylation of histone H3 lysine 4 (H3K4me3) accumulates at promoters in a gene activity dependant manner. The Set1 complex is responsible for most H3K4me3 in somatic cells and contains the conserved subunit Cfp1, which is implicated in targeting the Set1 complex to CpG islands in mammals. In mou...
ORGANISM(S): Mus musculus 
2014-08-20 | E-GEOD-53489 | biostudies-arrayexpress
UnknownTargeted Protein Acetylation in Cells Using Heterobifunctional Molecules.
Not available
S-EPMC10793965 | biostudies-literature
ProteomicsChronic alcohol consumption reprograms hepatic metabolism through organelle-specific acetylation in mice.
Post-translational acetylation of proteins at lysine side chains by the central metabolite acetyl-CoA, is a crucial regulator of proteostasis. Ethanol metabolism in the liver induces protein acetylation and disrupts hepatic substrate metabolism. While acetylation can influence gene transcription, en...
ORGANISM(S): Mus musculus (Mouse) 
2025-08-04 | PXD055349 | Pride
Proteomics Acetylation Liver Alcohol Lc-ms/ms 
TranscriptomicsH3K9acetyl ChIP on chip for integrative epigenomic study in leukemia samples
The molecular heterogeneity of acute leukemias and other tumors constitutes a major obstacle towards understanding disease pathogenesis and developing new targeted-therapies. Aberrant gene regulation is a hallmark of cancer and plays a central role in determining tumor phenotype. We predicted that ...
ORGANISM(S): Homo sapiens 
2010-05-22 | E-GEOD-10887 | biostudies-arrayexpress
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