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MetabolomicsThermal Profiling Maps Multilayered Organization of the Mitochondrial Proteome

Mitochondria execute essential metabolic, biosynthetic, and signaling functions, yet the regulatory principles governing their proteome remain incompletely understood. Here, we develop a thermal proteome profiling (TPP) workflow applied directly to purified mitochondria, enabling systematic analy...

2025-12-08 | MTBLS7386 | MetaboLights
ProteomicsThermal proteome profiling on zebrafish using napabucasin
In this project we performed thermal proteome profiling experiments on zebrafish embryos with the drug napabucasin. The lysate was heat treated, TMT labelled and subsuquently high pH fractionated.
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2020-10-29 | PXD017419 | Pride
Thermal proteome profiling Tmt Zebrafish 
ProteomicsOff-target profiling of Olaparib using Thermal Proteome Profiling
Explore off-targets of Olaparib using 2D Thermal Proteome Profiling (2D-TPP)
ORGANISM(S): Homo sapiens (Human) 
2024-05-13 | PXD046614 | Pride
Thermal proteome profiling Parp 2d-tpp Olaparib 
ProteomicsDanio rerio thermal proteome profiling with pervanadate as drug of interest
In this study we performed thermal proteome profiling experiments on zebrafish lysate after treatment with the drug pervanadate.
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2020-10-29 | PXD017418 | Pride
High ph Tmt Danio rerio Thermal proteome profiling 
ProteomicsThermal proteome profiling of phosphorylated proteoforms
We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD015993 | Pride
Phosphopeptides Meltome Phosphoproteomics Thermal proteome profiling 
ProteomicsThermal proteome profiling in Escherichia coli
We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-07-09 | PXD009495 | Pride
Target engagement Escherichia coli Drugs Growth phase Meltome Thermal proteome profiling 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive proteome: temperature range thermal profiling experiment
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033713 | Pride
Toxoplasma Parasite Calcium signaling Cetsa Tpp Apicomplexan Systems biology Thermal profiling 
MetabolomicsResolving coral photoacclimation dynamics through coupled photophysiological and metabolomic profiling
Corals continuously adjust to short term variation in light availability on shallow reefs. Long-term light alterations can also occur due to natural and anthropogenic stressors, as well as management interventions such as coral transplantation. Although short term photophysiological responses are re...
2019-04-11 | MTBLS768 | MetaboLights
ProteomicsThermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)
Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extra...
ORGANISM(S): Toxoplasma gondii GT1 
2020-07-13 | PXD018810 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa 
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