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MetabolomicsThermal Profiling Maps Multilayered Organization of the Mitochondrial Proteome

Mitochondria execute essential metabolic, biosynthetic, and signaling functions, yet the regulatory principles governing their proteome remain incompletely understood. Here, we develop a thermal proteome profiling (TPP) workflow applied directly to purified mitochondria, enabling systematic analy...

2025-12-08 | MTBLS7386 | MetaboLights
MetabolomicsDoes Storage Post Sampling Influence the Stability of Sebum when used for Mass Spectrometry Metabolomics Analysis?

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sebum sampling is facile and non-invasive, its potential for use in clinical bioc...

2025-07-15 | MTBLS7946 | MetaboLights
MetabolomicsThe stability and functional redundancy of a microbial cheese ecosystem subjected to a biotic perturbation
The cheese microbial community is dense with relatively low complexity and composed of cultivable prokaryotic and eukaryotic microorganisms. The ripening process can thus be reproduced under controlled conditions. A reduced microbial community composed of yeasts and bacteria was assembled to mimic t...
2024-04-29 | MTBLS6968 | MetaboLights
MetabolomicsCoral symbiotic bacterial community and metabolites changes under thermal stress: A case of laboratory experiment with heating treatment
Coral bleaching and coral reef degradation become severe as the surface seawater temperature rises. Much research to date has focused on the bacterial community composition properties within the coral holobiont, but less attention has been paid to the interactions of bacteria and corals under therma...
2022-10-27 | MTBLS5272 | MetaboLights
ProteomicsIdentification of phosphosites that alter protein thermal stability
We developed a robust method termed Dali that identifies phosphorylation events that alter protein thermal stability as a proxy for function.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-18 | PXD016750 | Pride
Proteomics Function Thermal stability Phosphorylation Dali 
ProteomicsIntegrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.
Altered thermal solubility measurement techniques are emerging as powerful tools to assess ligand binding, post-translational modification, protein-protein interactions, and many other cellular processes that affect protein state under various cellular conditions. Thermal solubility or stability pro...
ORGANISM(S): Escherichia coli 
2024-02-14 | PXD031800 | Pride
Pisa Thermal proteomic profiling 
ProteomicsThermal proteome profiling of cell cycle
We combined quantitative mass spectrometry with thermal profiling to systematically analyze the thermal stability and solubility of proteins during the eukaryotic cell cycle on a proteome-wide scale.
ORGANISM(S): Homo sapiens (Human) 
2018-05-02 | PXD008646 | Pride
Complexes Disordered proteins Cell cycle Thermal stability 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsDECODING PROTEIN METHYLATION FUNCTION WITH THERMAL STABILITY ANALYSIS
Protein methylation is emerging as an important modification beyond epigenetics. However, systems-wide analyses of protein methylation function lag behind compared to other modifications. Recently, thermal stability analyses have been developed which provide a proxy of a protein functional status. H...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-27 | PXD038939 | Pride
Thermal stability Protein methylation Mouse embryonic stem cells Prmt5 Ezh2 Pisa 
ProteomicsInvestigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling
Heat shock proteins are responsible for protein folding in cells. HSP90 is one of the most important chaperones in human cells, and inhibiting HSP90 is a potential strategy for cancer therapy. Multiple HSP90 inhibitors have been in clinical trials. However, none of them has been approved for disease...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037575 | Pride
Heat shock protein 90 Hsp90 inhibitors And protein abundance change Thermal proteome profiling Cell response Protein thermal stability shift 
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