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ProteomicsIdentification of phosphosites that alter protein thermal stability
We developed a robust method termed Dali that identifies phosphorylation events that alter protein thermal stability as a proxy for function.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-18 | PXD016750 | Pride
Proteomics Function Thermal stability Phosphorylation Dali 
ProteomicsThermal proteome profiling of cell cycle
We combined quantitative mass spectrometry with thermal profiling to systematically analyze the thermal stability and solubility of proteins during the eukaryotic cell cycle on a proteome-wide scale.
ORGANISM(S): Homo sapiens (Human) 
2018-05-02 | PXD008646 | Pride
Complexes Disordered proteins Cell cycle Thermal stability 
ProteomicsIntegrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.
Altered thermal solubility measurement techniques are emerging as powerful tools to assess ligand binding, post-translational modification, protein-protein interactions, and many other cellular processes that affect protein state under various cellular conditions. Thermal solubility or stability pro...
ORGANISM(S): Escherichia coli 
2024-02-14 | PXD031800 | Pride
Pisa Thermal proteomic profiling 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsInvestigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling
Heat shock proteins are responsible for protein folding in cells. HSP90 is one of the most important chaperones in human cells, and inhibiting HSP90 is a potential strategy for cancer therapy. Multiple HSP90 inhibitors have been in clinical trials. However, none of them has been approved for disease...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037575 | Pride
Heat shock protein 90 Hsp90 inhibitors And protein abundance change Thermal proteome profiling Cell response Protein thermal stability shift 
ProteomicsDECODING PROTEIN METHYLATION FUNCTION WITH THERMAL STABILITY ANALYSIS
Protein methylation is emerging as an important modification beyond epigenetics. However, systems-wide analyses of protein methylation function lag behind compared to other modifications. Recently, thermal stability analyses have been developed which provide a proxy of a protein functional status. H...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-27 | PXD038939 | Pride
Thermal stability Protein methylation Mouse embryonic stem cells Prmt5 Ezh2 Pisa 
ProteomicsMitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
Cellular functionality relies on a well-balanced, but highly dynamic proteome. Dysfunctionof mitochondrial protein import leads to the cytosolic accumulation of mitochondrial precursor proteins which compromise cellular proteostasis and trigger the mitoprotein-induced stress response. To dissect the...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-21 | PXD037741 | Pride
Mitochondria / proteasome / protein folding / proteostasis / thermal proteome profiling / stress response 
ProteomicsMeltome Atlas - thermal proteome stability across the tree of life
We have developed a mass spectrometry-based approach that allowed us to quantitatively monitor protein stability across a broad range of temperatures at the proteome scale (meltome). We profiled the meltomes of several microorganisms and eukaryotic species including human, allowing to investigate th...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Picrophilus torridus Oleispira antarctica Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Drosophila melanogaster (Fruit fly) Caenorhabditis elegans Geobacillus stearothermophilus Homo sapiens (Human) Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) Bacillus subtilis 
2020-04-29 | PXD011929 | Pride
Protein stability Thermostability Meltome atlas Melting point Thermal proteome profiling 
ProteomicsExperimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays
Mass spectrometry (MS)-based thermal stability assays have recently emerged as one of the most promising solutions for the identification of protein-ligand interactions. Here, we have investigated eight combinations of several recently introduced MS-based advancements, including the Phase-Constraine...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD040284 | Pride
Cetsa Mass spectrometry Tpp Target identification Thermal stability assays Drug-protein interactions 
ProteomicsInvestigation of CDK substrate thermal stability in Jurkat T cells
In order to determine how phosphorylation of CDK substrates affects their thermal stability, we treated Jurkat T cells with DMSO, Nocodazole, or Nocodazole + CDK inhibitors (Dinaciclib and Palbociclib).
ORGANISM(S): Homo sapiens (Human) 
2024-06-17 | PXD050445 | Pride
Cdk Thermal stability Jurkat t cells 
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