Sort   by:  
 Page size 
UnknownCombining high-energy C-trap dissociation and electron transfer dissociation for protein O-GlcNAc modification site assignment.
Not available
S-EPMC3172619 | biostudies-literature
ProteomicsO-Fucosylation of CPN20 by SPINDLY Derepresses Abscisic Acid Signaling during Seed Germination and Seedling Development--Partial Submission
We utilized ETD (Electron Transfer Dissociation)-MS/MS analysis to identify O-fucosylation sites in ectopically expressed CPN20 in mammalian cells and Arabidopsis.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-17 | PXD027272 | Pride
O-fucosylation Etd Lc-ms/ms Cpn20 
ProteomicsThe E. coli effector EspJ blocks Src kinase activity via amidation and ADP-ribosylation
In vitro ADP-ribosylated Src-K295M was separated by SDS-PAGE and the Coomassie stained protein band excised and digested with either trypsin, thermolysin or elastase. All digests were analysed by nanoLC-MS/MS using Higher-energy C-trap Dissociation (HCD) as well as Electron-Transfer Dissociation (ET...
ORGANISM(S): Homo sapiens (Human) 
2014-12-19 | PXD001461 | Pride
Amidation Src kinase Adp-ribosylation Espj 
ProteomicsSequencing of monoclonal antibody 1D3 (mAb1D3)
De novo sequencing and expression of recombinant 1D3 antibody (R1D3). To achieve maximum coverage for antibody sequencing, 5 µg of the antibody original 1D3 was digested in parallel with five different proteases: trypsin, elastase, chymotrypsin, and Asp-N. For each digestion mixture, peptides were l...
ORGANISM(S): Homo Sapiens (human) 
2022-09-25 | PXD036958 | JPOST Repository
ProteomicsEntamoeba histolytica splicing/mRNP proteome
First, the snRNP component U1A was HA-tagged and the intron of the RabX13 gene was MS2-tagged and amoeba transformats were established with such constructs. Next, amoeba transformants were UV cross-linked and their nucler extracts were immunoprecipitated (IP) with anti-HA agarose or MS2-spharose bea...
ORGANISM(S): Entamoeba histolytica 
2014-09-11 | PXD001080 | Pride
Clip Entamoeba histolytica U1a Thermo electron velos orbitrap etd 
ProteomicsCharacterisation of protein methyltransferases Rkm1, Rkm4, Efm4, Efm7, Set5 and Hmt1 reveals extensive post-translational modification
Protein methylation is one of the major post-translational modifications (PTMs) in the cell. In Saccharomyces cerevisiae, over twenty protein methyltransferases (MTases) and their respective substrates have been identified. However, the way in which these MTases are modified, and potentially subject...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-12-18 | PXD006767 | Pride
Metyltransferases Post-translational modifications Rkm1 Hmt1 Efm7 Rkm4 Set5 Efm4 
ProteomicsIntegrative genome, transcriptome and proteome analysis of rat livers from two different genetic backgrounds
In this project, we aim to pair-wise analyze the genomes, transcriptomes and proteomes of in-bred rats originating from two different genetic backgrounds. These two strains are Brown Norway (BN-Lx) and Spontaneously Hypertensive Rats (SHR). First, we re-sequenced the genomes for both BN and SHR rats...
ORGANISM(S): Rattus norvegicus (Rat) 
2013-11-29 | PXD000131 | Pride
Lc-msms Rat Proteome Livers 
MetabolomicsIntegrated omics analysis of pathogenic host responses during pandemic H1N1 influenza virus infection: the crucial role of lipid metabolism
Pandemic influenza viruses modulate pro-inflammatory responses that can lead to immunopathogenesis. We present an extensive and systematic profiling of lipids, metabolites and proteins in respiratory compartments of ferrets infected with either 1918 or 2009 human pandemic H1N1 influenza viruses. Int...
2016-05-11 | MTBLS196 | MetaboLights
ProteomicsAnalysis of the extracellular proteome from Aspergillus nidulans growing in Avicel, sugarcane bagasse and sugarcane straw
Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes found in viruses, archaea, bacteria as well as eukaryotes, such as fungi, algae and insects, actively contributing to the degradation of different polysaccharides. Analysis of the extracellular proteome (secretome) from Aspergillus ni...
ORGANISM(S): Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) 
2022-05-09 | PXD031886 | Pride
Sugarcane lignocellulose Oxidative enzymes Carbohydrate metabolism Lpmo Aa9 Fungal biology 
ProteomicsIdentification of O-GlcNAcylated proteins and mapping of O-GlcNAc sites using Click Chemistry following extensive fractionation of extracted proteins
The O-N-acetyl-β-D-glucosaminylation, termed O-GlcNAcylation, is an atypical glycosylation corresponding to the transfer of a unique saccharide, the N-acetyl-β-D-glucosamine, on the hydroxyl group of serine and threonine amino acids of nuclear, cytosolic and mitochondrial proteins. Because of its dy...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-21 | PXD004860 | Pride
Muscle Mouse O-glcnacylation Lc_msms Sites mapping Post-translational modifications C2c12 muscle cells 
Sort   by:  
 Page size