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MetabolomicsHigh-confidence untargeted lipidomics of mammalian serum using LC-TIMS-PASEF

This study aimed to establish and validate a robust untargeted lipidomics workflow for the analysis of human serum using reversed-phase liquid chromatography coupled to trapped ion mobility spectrometry time-of-flight mass spectrometry (RP-LC-TIMS-TOF MS). Commercial pooled human serum was used a...

2026-05-26 | MTBLS14002 | MetaboLights
ProteomicsIon mobility fractionation coupled to PASEF for enhanced proteome coverage
Data provided report the use of trapped ion mobility spectrometry (TIMS) to fractionate ions in the gas phase based on their ion mobility (V⋅s/cm2) followed by parallel accumulation serial fragmentation (PASEF) in a quadrupole time-of-flight instrument. TIMS fractionation coupled to DDA-PASEF allowe...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033129 | Pride
Timstof pro Pasef Dia Segmented Tims fractionation Fractionation Optimized Deep coverage Dda Ion mobility 
UnknownEnhancement of Proteome Coverage by Ion Mobility Fractionation Coupled to PASEF on a TIMS-QTOF Instrument.
Not available
S-EPMC10653119 | biostudies-literature
ProteomicsOnline parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer
In bottom-up proteomics, peptides are separated by liquid chromatography with elution peak widths in the range of seconds, while mass spectra are acquired in about 100 microseconds with time-of-fight (TOF) instruments. This allows adding ion mobility as a third dimension of separation. Among several...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-11-06 | PXD010012 | Pride
Tims Pasef Technological 
ProteomicsRapid and ion mobility-resolved phosphoproteomics with dia-PASEF
Mass spectrometry-based phosphoproteomics has identified >150,000 post-translational phosphorylation sites in the human proteome. To disentangle their functional relevance, complex experimental designs that require increased throughput are now coming into focus. Here, we apply dia-PASEF on a trapped...
ORGANISM(S): Homo sapiens (Human) 
2022-10-26 | PXD033904 | Pride
Tims Technology Pasef Dia-pasef Phosphoproteomics Data-independent acquisition Ion mobility 
ProteomicsSynchro-PASEF slices peptide precursors enabling specific fragment extraction and interference removal in data independent acquisition
Data independent acquisition methods have become increasingly popular in mass spectrometry (MS)-based proteomics because they enable parallel and continuous acquisition of fragment spectra. However, these advantages come with the challenge of correctly reconstructing the precursor-fragment relations...
ORGANISM(S): Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) 
2023-03-10 | PXD037766 | Pride
Pasef Tof Rapid analysis Tims Data-independent acquisition Scan mode 
ProteomicsRapid and in-depth coverage of the (phospho-)proteome with deep libraries and optimal window design for dia-PASEF
Data-independent acquisition (DIA) methods have become increasingly attractive in mass spectrometry (MS)-based proteomics, because they enable high data completeness and a wide dynamic range. Recently, we combined DIA with parallel accumulation – serial fragmentation (dia-PASEF) on a Bruker trapped ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-11 | PXD034128 | Pride
Pasef Egf Tims Data-independent acquisition Phosphoproteomics Systems biology 
Proteomicsdia-PASEF analysis of human amygdala in Alzheimer’s disease
dia-PASEF analysis of human amygdala in Alzheimer’s disease
ORGANISM(S): Homo sapiens (Human) 
2023-06-28 | PXD038322 | Pride
Dia-pasef Alzheimer Human Amygdala 
ProteomicsTIMS²Rescore: A DDA-PASEF optimized data-driven rescoring pipeline based on MS²Rescore - Plasma proteomics
The high throughput analysis of proteins with mass spectrometry (MS) is highly valuable for understanding human biology, discovering disease biomarkers, identifying therapeutic targets, and exploring pathogen interactions. To achieve these goals, specialized proteomics subfields – such as plasma pro...
ORGANISM(S): Homo sapiens (Human) 
2025-02-17 | PXD053748 | Pride
Plasma Data-driven rescoring 
UnknownTrapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts.
Not available
S-EPMC6965134 | biostudies-literature
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