Timstof ultra 2

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Proteomics (249)
Metabolomics (15)
Unknown (3)
Other (1)

Organisms

Allexis (224)
Arabidopsis thaliana (224)
Homo sapiens (224)
Listeria monocytogenes (224)
Mus musculus (224)
Rattus norvegicus (224)
Saccharomyces cerevisiae (224)
Schizosaccharomyces pombe (224)
Acidithiobacillus ferrooxidans ATCC 53993 (223)
Aerococcus urinae NBRC 15544 = CCUG 36881 (223)
Candida albicans (223)
Cyanophora paradoxa (223)
Ensifer adhaerens (223)
Enterococcus faecalis (223)
Globicatella sanguinis (223)
Histomonas meleagridis (223)
Leishmania major strain Friedlin (223)
Lotus japonicus (223)
Medicago truncatula (223)
Methylohalobius crimeensis 10Ki (223)
Mycobacterium tuberculosis (223)
Oryza sativa Indica Group (223)
Physcomitrium patens (223)
Pseudomonas putida KT2440 (223)
Schizosaccharomyces pombe 972h- (223)
Solanum lycopersicum (223)
Solanum tuberosum (223)
Staphylococcus aureus (223)
Streptococcus dysgalactiae subsp. equisimilis SK1249 (223)
Mus musculus (1)

Repository

pride (224)
MassIVE (25)
MetaboLights (15)
iProX (1)

Tissue

Cell culture (45)
Brain (17)
Epithelial cell (7)
Blood plasma (6)
Permanent cell line cell (6)
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Colorectal adenocarcinoma cell line (2)
Early embryonic cell (2)
Endothelial cell of umbilical vein (2)
Epithelial cell of cervix (2)
Exoskeleton (2)
Heart (2)
HepG2 cell (2)
Hepatocyte (2)
JURKAT cell (2)

Disease

Alzheimer's disease (5)
Colon cancer (5)
Cardiovascular system disease (2)
Disease free (2)
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Human immunodeficiency virus infectious disease (2)
Psoriasis (2)
Acute leukemia (1)
Acute myocardial infarction (1)
Atherosclerosis (1)
Autosomal recessive congenital ichthyosis (1)
Bacterial infectious disease (1)
Brain glioblastoma multiforme (1)
Breast cancer (1)
Bronchopulmonary dysplasia (1)
COVID-19 (1)
Calcinosis (1)
Cancer (1)
Chronic traumatic encephalopathy (1)
Colorectal adenoma (1)
Colorectal cancer (1)
Corticobasal degeneration syndrome (1)
Crohn's disease (1)
Cutaneous lupus erythematosus (1)
Lewy body dementia (1)
Neurodevelopmental delay (1)
Parkinson's disease (1)
Pick's disease (1)
Sjogren's Syndrome (1)
Wounds and Injuries (1)

Technology Type

Mass Spectrometry (224)
Bottom-up proteomics (118)
Shotgun proteomics (70)
Data-dependent acquisition (53)
Data-independent acquisition (45)
DiaPASEF (13)
Mass spectrometry (9)
Affinity purification coupled with mass spectrometry proteomics (8)
Mass spectrometry assay (6)
SWATH MS (6)
Top-down proteomics (3)
Affinity proteomics (2)
Chemical cross-linking coupled with mass spectrometry proteomics (2)
PAcIFIC (1)
SRM/MRM (1)

Instrument Platform

Liquid Chromatography MS - positive - reverse phase (11)
Liquid Chromatography MS - negative - reverse phase (10)
TimsTOF Ultra (9)
TimsTOF Pro (7)
TimsTOF Ultra 2 (5)
TimsTOF HT (3)
TimsTOF fleX (3)
Liquid Chromatography MS - positive - hilic (3)
TimsTOF Pro 2 (2)
TimsTOF SCP (2)
Liquid Chromatography MS - positive - reverse-phase (2)
Liquid Chromatography MS - negative - reverse-phase (2)
Liquid Chromatography MS - negative - hilic (2)
Orbitrap Astral (1)
Orbitrap Eclipse (1)
Orbitrap Exploris 480 (1)
Orbitrap Fusion Lumos (1)
Q Exactive HF (1)
ZenoTOF 7600 (1)
Gas Chromatography MS - positive (1)

Publication Date

2025 (97)
2026 (63)
2024 (43)
2023 (32)
2022 (18)
2021 (8)
2020 (1)
2018 (1)

CHEBI ID

CHEBI:15741 (2)
CHEBI:17781 (2)
CHEBI:17015 (2)
CHEBI:16196 (2)
CHEBI:15940 (2)
CHEBI:16467 (2)
CHEBI:16015 (2)
CHEBI:18012 (2)
CHEBI:32816 (2)
CHEBI:17191 (2)
CHEBI:15603 (2)
CHEBI:17295 (2)
CHEBI:17351 (2)
CHEBI:18344 (2)
CHEBI:30769 (2)
CHEBI:137510 (1)
CHEBI:78346 (1)
CHEBI:57870 (1)
CHEBI:61548 (1)
CHEBI:58336 (1)
CHEBI:57642 (1)
CHEBI:176837 (1)
CHEBI:176836 (1)
CHEBI:176835 (1)
CHEBI:176834 (1)
CHEBI:176833 (1)
CHEBI:176832 (1)
CHEBI:176831 (1)
CHEBI:176830 (1)
CHEBI:139135 (1)

Metabolite Name

Oleoyl Ethanolamide (2)
O-acetyl-L-carnitine (2)
L-isoleucine (2)
N1-Acetylspermidine (2)
N1,N12-Diacetylspermine (2)
Indole (2)
Oleamide (2)
Sphinganine (2)
Level 5 (1)
PTM_1919 (1)
[4-phenyl-5-(trifluoromethyl)-4H-1,2,4-triazol-3-yl]methanamine (1)
[(1S,7S,8S,8aS)-8-[2-[(2R,4R)-4-hydroxy-6-oxooxan-2-yl]ethyl]-7-methyl-1,2,3,4,4a,7,8,8a-octahydronaphthalen-1-yl] (2S)-2-methylbutanoate (1)
Tenivastatin sodium (1)
Palmitoyl Carnitine (1)
Nadide Phosphate (1)
N-{1-[(6-ethoxy-3-pyridyl)methyl]tetrahydro-1H-pyrrol-3-yl}-2-(3-pyridyl)acetamide (1)
N-cyclohexyl-4-ethoxypiperidine-1-carboxamide (1)
N-Butylaniline (1)
N-(oxolan-3-ylmethyl)-N-propylcyclohexanecarboxamide (1)
N-(2-cyanoethyl)-2-phenylethylamine (1)
N-(2,4-difluorophenyl)-2-(1,1-dioxo-2H-1lambda6,2,4-benzothiadiazin-3-yl)acetamide (1)
N-(2,4-Difluorophenyl)-1-{[4-(pyrrolidine-1-carbonyl)thiophen-2-YL]sulfonyl}piperidine-4-carboxamide (1)
N,N-Diisopropylaniline (1)
N,N,N-trimethyl-2-(phosphonooxy)ethanaminium (1)
Methyl 3-[(2-methyl-1,3-thiazolidin-4-yl)formamido]propanoate (1)
Methyl 2-{[2-(4-methylphenyl)azepane-1-carbonyl]amino}acetate (1)
Methionylglutamic acid (1)
Lovastatin Hydroxy Acid (1)
Leu-Glu-Gly (1)
Leu-Asp-Cys (1)

Release Date

2025 (2)
2024 (1)

Lab affiliation

School of Life Sciences, Central China Normal University, Wuhan, Hubei province, China (13)
Institute of Microbiology - BioCeV, Academy of Sciences of the Czech Republic (7)
Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST) (3)
Department of Neurobiology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA (3)
Institute of Microbiology of the CAS (3)
MS Omics, Biohub Chicago, USA (3)
Associate Professor of Systems Biology Department of Molecular Medicine University of Pavia 9, via A Ferrata/ 27100 Pavia / Italy (2)
Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Canada (2)
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China; (2)
Chemical and Biological Engineering, Hong Kong University of Science and Technology (HKUST) (2)
Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST) (2)
Department of Molecular Biology, Princeton University (2)
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic (2)
Escola Superior de Agricultura Luiz de Queiroz da Universidade de São Paulo (2)
International Institute of Molecular and Cell Biology, Laboratory of RNA-Protein Interactions - Dioscuri Centre, Warsaw, Poland (2)
Lee Kong Chian School of Medicine, School of Biological Sciences, Nanyang Technological University, Singapore (2)
MSBioLMU Großhaderner Str. 2-4 82152 Planegg-Martinsried (2)
Max Delbrück Center Robert-Rössle-Straße 10 13125 Berlin, Germany (2)
Systems Arbovirology, Leibniz Institute of Virology, Hamburg, Germany (2)
Tilden-Weger-Bieler Professor of Medicine Chief of Preventive Medicine & Nutrition Columbia University (2)
University of California Los Angeles (2)
University of South Florida (2)
University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry (2)
1 - College of Pharmacy, University of Sharjah, Sharjah 2 - Sharjah Institute for Medical Research, University of Sharjah, Sharjah (1)
1. Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Laboratory of Plant Biophysics & Biochemistry, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic 2. University of South Bohemia, Faculty of Science, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic (1)
1Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA 2Institute for Quantitative and Computational Biosciences, University of California, Los Angeles, Los Angeles, CA, USA 3Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA (1)
1、Capital Institute of Pediatrics,Chinese Academy of Medical Sciences & Peking Union Medical College 2、Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics (1)
95 Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, Université Toulouse III - Paul Sabatier (UT3), Toulouse, 31077, France (1)
Aalborg University, Denmark (1)
Aix-Marseille Université, CEA, CNRS, Institute of Biosciences and Biotechnologies of Aix-Marseille, UMR 7265, CEA Cadarache, Saint-Paul-lez Durance F-13108, France (1)

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The $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers

Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To democratize access to proteomics and facilitate its integration into more high-throughput multiomic...

ORGANISM(S): Homo sapiens (Human)

2026-02-04 | PXD066701 | Pride

Limited sample input $10 proteome

Regulatory Role of the VCL/ICAM-1 Pathway in Inflammatory Exudative Damage to the Gas-Blood Barrier in SARS-CoV-2 Infection: New Insights from the Omicron Paradigm

Infections by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), ranging from the initial Wildtype (WT) variant in Wuhan, China, to later variants including Omicron, have showcased a diverse spectrum of symptoms. This diversity underscores the need for ongoing exploration into the vir...

2025-02-28 | MTBLS12231 | MetaboLights

The $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers.

Not available

S-EPMC12324313 | biostudies-literature

PASEF technology on timsTOF Pro improves quality of FPOP analysis

Fast photochemical oxidation of proteins (FPOP) is a modern technique for studying protein folding, conformations, interactions, etc. In this work, timsTOF Pro mass spectrometer coupled with trapped ion mobility spectrometry was used for identification of oxidative modifications in a model protein. ...

ORGANISM(S): Homo sapiens (Human)

2021-06-10 | PXD020509 | Pride

Tims Pasef Timstof Protein footprinting Hydroxyl radical Fpop

Proteomic Analysis of Human Conjunctival Cells and Tears Collected with Schirmer Strips Using TimsTOF Pro

This study aimed to investigate the human tear proteins extracted from whole (W) Schirmer strips (ScS) and their two parts, the bulb (B) and the rest of the strip (R) with a comprehensive proteomic approach using a trapped ion mobility mass spectrometer, the timsTOF Pro. Eight ScS were collected fro...

ORGANISM(S): Homo sapiens (Human)

2022-01-27 | PXD030334 | Pride

Proteomics Conjunctival cells Schirmer strips Tears Timstof pro

Single cell proteomics of hepatocytes derived from patients with liver disfunction

Single human hepatocytes were derived from patients with normal liver function and deleterious mutations that often lead to liver cancer. Single cells and bulk libraries of 25 hepatocytes were isolated using a Tecan UNO single cell printer. Cells were lysed and digested in plate before being loaded ...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

2025-08-19 | MSV000098879 | MassIVE

Study of molecular mechanisms in lung injury due to ultra-high and conventional dose rate pulsed radiation based on 4D-DIA proteomics

Objective: To investigate the mechanism of ultra-high dose rate pulsed radiation in radiation-induced lung injury (RILI), providing an experimental and theoretical basis for the application of FLASH-RT in radiotherapy. Methods: C57BL/6J mice were randomly divided into three groups: a sham group, a F...

ORGANISM(S): Mus musculus (Mouse)

2025-05-07 | PXD057784 | Pride

Radiation-induced lung injury ultra-high dose rate flash radiotherapy fibrosis flash effect

Comparison of popular enrichment methods for untargeted in vitro metabolomics.

Introduction

Untargeted metabolomics is a popular method by which researchers measure a large portion of the metabolites present in a biological system at once. This approach usually results in complex data sets containing tens to hundreds of thousands of observations whi...

2025-08-11 | MTBLS12332 | MetaboLights

Phosphoproteomics of Natural Killer (NK) Cells Stimulated with Fibrin and IL-15

Phosphoproteomic analysis of fibrin or IL-15 treated NK cells with timsTOF HT

ORGANISM(S): Homo sapiens (Human)

2024-06-26 | PXD049692 | Pride

Timstof ht Nk cells Phoshoproteomics

What does next generation Mass Spectrometry offer for proteomics? A comprehensive platform comparison

Next-generation mass spectrometry platforms (Orbitrap Astral, timsTOF Ultra) are reshaping proteomics by enhancing analytical depth and sensitivity. We compared these platforms against Orbitrap Exploris 480 using neonatal mouse lung tissue from a bronchopulmonary dysplasia model (n=12), employing fo...

ORGANISM(S): Mus musculus (Mouse)

2026-04-02 | PXD057442 | Pride

Mouse Lung Bronchopulmonary dysplasia Platform comparison

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268 Results

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Introduction

OmicsDI is part of the ELIXIR infrastructure

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