Being inspired by metabolomic data processing, we have developed a bioinformatic pipeline that optimizes the processing of mass spectral data obtained from isobaric Tandem Mass Tag (TMT) experiments. Our method focuses on the tandem mass spectral level by first quantifying and then identifying (QtI)...
ORGANISM(S): Homo sapiens (Human) Escherichia coli
This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.
The change in the plasma proteome in chicken (Gallus gallus) challenged with lipopolysaccharide (endotoxin) derived from E. coli was determined by relative quantitation using a TMT label-based approach. This study provides insights into different factors contributing to innate immune response to exp...
Mapping the subcellular proteome using a differential fractionation and an orthogonal nycodenz fractionation combined with quantitative mass spectrometry using isobaric labeling (TMT) in MS2
Cardiovascular-related disorders (CVD) account for the highest mortality rates in the world, and affect the quality and the quantity of life of patients, creating an economic burden of prolonged therapeutic intervention. The aim of this study was to evaluate the serum protein profile of healthy and ...
Bovine mastitis, the infection of the mammary gland which leads to great health and economic challenges for dairy farmers is accompanied by dramatic changes in the milk proteome. In this study of naturally occurring mastitis not only have the changes in the milk proteome been quantified in subclini...
Chronic skeletal muscle mitochondria dyshomeostasis drives tubular aggregate formation. TMT sixplex Isobaric Mass Tagging analysis was carried out in the CBMSO Protein Chemistry Facility (ProteoRed, PRB3-ISCIII and UAM University, Spain.
TMT sixplex Isobaric Mass Tagging (TAM) analysis was carried out in the CBMSO Protein Chemistry Facility (ProteoRed, PRB3-ISCIII and UAM University, Spain. Chronic skeletal muscle mitochondria dyshomeostasis drives tubular aggregate formation.
