Sort   by:  
 Page size 
ProteomicsProtein and peptide turnover measurements using pulsed SILAC-TMT approach
We evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved in...
ORGANISM(S): Homo sapiens (Human) 
2018-02-09 | PXD008579 | Pride
Degradation rates Protein turnover Proteoforms Posttranslational cleavage Synthesis rates Tmt Splice variants Pulsed silac Posttranslational modification 
ProteomicsTranslatome Hypoxia-Acidosis, TMT-pulse SILAC
Cells were grown in light (R0K0) SILAC media (AthenaES) for 7 days, they were then incubated in light media for 24 hours in respective condition (normoxia neutral (NN) pH: 7.4; hypoxia neutral (HN)1% O2, pH: 7.4; hypoxia acidosis (HA), 1% O2, pH=6) and pulsed with heavy (R10K8) SILAC media (AthenaES...
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD015643 | Pride
U87-mg Tmt-pulse silac 
ProteomicsLinking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling
Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labelling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated and u...
ORGANISM(S): Homo sapiens (Human) 
2021-11-10 | PXD023218 | Pride
Human Ptms Turnover Silac 
ProteomicsSystematic analysis of proteome turnover in an organoid model of pancreatic cancer by dSILO
The role of protein turnover in pancreatic ductal adenocarcinoma (PDA) metastasis has not previously been investigated. We introduce dynamic Stable-Isotope Labeling of Organoids (dSILO): a dynamic SILAC derivative that combines a pulse of isotopically labeled amino acids with isobaric tandem-mass t...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-07-21 | MSV000092498 | MassIVE
ProteomicsPulsed SILAC study of cell lines following PPIA knockdown
Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spec...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-16 | MSV000093125 | MassIVE
UnknownPeptide Level Turnover Measurements Enable the Study of Proteoform Dynamics.
Not available
S-EPMC5930408 | biostudies-literature
ProteomicseIF4A1 unwinding activity is RNA sequence-specifically deployed through eIF4A1-multimerisation to facilitate translation of mRNAs with local repressive RNA structure
Oncogenic translational programmes are an emerging hallmark of cancer and often driven by dysregulation of signaling pathways including KRAS and mTORC that converge on the eukaryotic translation initiation (eIF) 4F complex. Altered eIF4F activity promotes translation of oncogene mRNAs that typically...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD034343 | Pride
Proteomics Tmt Eif4a1 Pulse silac 
ProteomicsProteome-wide pulse labeling reveals amyloid-impaired protein degradation in Alzheimer’s disease
We here present a large-scale proteome-wide profiling of protein turnover in Alzheimer's disease mice model. We integrated in vivo pulse-SILAC labeling and TMT multiplexing with novel mass spectrometry to precisely measure protein half-lives.
ORGANISM(S): Mus musculus (Mouse) 
2024-10-28 | PXD031830 | Pride
Alzheimer’s disease Tmt Protein turnover Sialc 
ProteomicsTranslatome and Translation Machinery Remodeling by eIF4A Inhibitors Mediates Antitumor Potency.
Protein synthesis-targeting agents against eIF4A activity, including rocaglates, are actively pursued as anticancer and antiviral therapies. Yet, their full effect on the translational landscape is unknown, especially with regards to up-regulated proteins and drug-activated translation factors that ...
ORGANISM(S): Homo sapiens (Human) 
2021-11-23 | PXD022556 | Pride
Cancer Eiffa Matrix Translatome Rocaglate Pulse silac Translate 
ProteomicsEvaluation of deep and dynamic proteomic screening strategies at sub-50Hz scan rate and without automation
In this study, we systematically evaluate sample preparation strategies, data acquisition modes and data-analysis approaches for both labeled and label-free proteomics, with the explicit goal of maximizing throughput and quantitative depth using Orbitrap-based instruments operating below 50Hz scan r...
ORGANISM(S): Homo sapiens (Human) 
2026-03-27 | PXD073468 | Pride
Sort   by:  
 Page size