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GenomicsTMT_proteomics_Ctrl
Ctrl samples: TMT-proteomics using Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer, TMT10plex (N=8 sample batch) or TMT16plex (2x N=16 sample batches) Isobaric Label Reagent (Thermo Fisher Scientific, Waltham, MA, USA), experiment type: bottom-up proteomics; data-dependent acquisition.
EGAD00010002702 | EGA
GenomicsTMT_proteomics_CUD
CUD samples: TMT-proteomics using Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer, TMT10plex (N=8 sample batch) or TMT16plex (2x N=16 sample batches) Isobaric Label Reagent (Thermo Fisher Scientific, Waltham, MA, USA), experiment type: bottom-up proteomics; data-dependent acquisition.
EGAD00010002703 | EGA
ProteomicsNucleophosmin sustains WNT-driven cell proliferation and tumour initiation in vivo
Nucleophosmin (NPM1) is a nucleolar protein frequently mutated in haematopoietic malignancies and overexpressed in many solid tumours, but little is known about its functional role in carcinogenesis. Here, we identify NPM1 as a commonly upregulated gene following APC loss in tissues that positively ...
ORGANISM(S): Mus musculus (Mouse) 
2025-08-12 | PXD062969 | Pride
Nucleophosmin Wnt signalling Tmt16plex Colorectal cancer 
ProteomicsPCBP1 regulates aberrant alternative splicing of AARS2 in congenital cardiomyopathy
Alanyl-transfer RNA synthetase 2 (AARS2) is a nuclear encoded mitochondrial tRNA synthetase that is responsible for charging of tRNA-Ala with alanine during mitochondrial translation. Homozygous or compound heterozygous mutations in the Aars2 gene, including those affecting its splicing, are linked ...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-16 | PXD057114 | Pride
ProteomicsIdentifying putative substrates of Lon protease in Pseudomonas aeruginosa
We aim to identify potential substrates of the Lon protease in P. aeruginosa. Samples with the lon protease overexpressed or deleted were compared to the wild type to observe the proteins whose steady-state levels changed with change in Lon levels. We used spectinomycin to inhibit protein synthesis ...
ORGANISM(S): Bacteria Pseudomonas aeruginosa PA1 
2025-06-13 | PXD063095 | Pride
Proteomics Lon protease Bacteria Pseudomonas aeruginosa Substrates 
ProteomicsSenescence-specific translation dysregulation desensitizes cells to stress by inhibiting activation of the integrated stress response
IMR90 human fibroblasts were used to generate etoposide-induced senescent cells (Etop), contact-inhibited quiescent cells (Qui), and cycling cells (Cyc). Briefly, Etop cells were prepared with three treatments of 50 uM etoposide over 10 days, Qui cells were incubated for four days after 100% conflue...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-01-17 | MSV000091077 | MassIVE
ProteomicsThermal proteome profiling of sponge whole-body movement
Sponges (Porifera) are early-branching Metazoa who do not possess muscles or neurons, however are able to undergo a whole-body movement that involves the closure of their canal system and collapse of an epithelial tent. In this study we profile proteomic responses of the freshwater sponge Spongilla ...
ORGANISM(S): Spongilla lacustris 
2024-01-04 | PXD044169 | Pride
Spongilla Tpp Actomyosin Evolution 
ProteomicsA new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
Biological membranes have a stunning ability to adapt their composition in response to physiological stress and metabolic challenges. Little is known how such perturbations affect individual organelles in eukaryotic cells. Pioneering work provided insights into the subcellular distribution of lipids...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-03-05 | PXD048553 | Pride
Organelle isolation Proteomics Upr Lipid bilayer stress Lipidomics Membrane 
ProteomicsQuantitaQuantitative proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction tive proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction
We designed a TMT peptide labeling-based quantitative proteomics strategy to achieve a high-accuracy analysis of proteomic changes in response to ER stress and mitochondria stress on INS-1 cells under three different culture conditions: control (Cont), rotenone (Rot), and thapsigargin (Tpg), each wi...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-07-20 | PXD030711 | Pride
Endoplasmic reticulum (er) stress  mitochondria stress  quantitative proteomics  tmtpro  ins-1 beta cells Cells  metascape 
ProteomicsQuantitative TMT-MS of HPV42, HPV16 or empty vector transduced keratinocytes.
The skin serves as a barrier to the environment and is constantly exposed to viral pathogens. Whether viral pathogens contribute to the oncogenesis of rare skin cancers has not been systematically explored. Here, we analyzed 18 skin cancer types by exploiting off-target reads from commonly available...
ORGANISM(S): Homo sapiens (Human) 
2022-10-18 | PXD031764 | Pride
Keratinocytes Human Tmt-ms Hpv 
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