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ProteomicsA genetic model for in vivo proximity labeling of the mammalian secretome
Yang R, Meyer AS, Droujinine I, Udeshi ND, Hu Y, Guo J, McMahon J, Carey DK, Xu C, Qiao F, Sha J, Qin S, Rocco D, Wohlschlegel J, Ting AY, Carr SA, Perrimon N, McMahon AP. 2022. Organ functions are highly specialized and interdependent. Tissue-specific secreted factors play a critical role in organ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-02-16 | MSV000088848 | MassIVE
ProteomicsTMT6plex quantitative proteomics of 293T NME4 KO cell
To identify the role NME4,we knockout NME4 in 293T cells. Followed by protein extraction, trypsin digestion, TMT6 labeled, HPLC fractionation and LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD036628 | Pride
Human nme4 tmt6 
ProteomicsTime-resolved analysis of proteome dynamics by TMT-SILAC hyperplexing
In this project, a more efficient methodology for obtaining multi-timepoint kinetic data for proteome-wide analyses of protein turnover is developed and used to globally measure the kinetics of protein turnover in primary human fibroblasts (HCA2-hTert). This approach takes advantage of the multiplex...
ORGANISM(S): Homo sapiens (Human) 
2016-12-14 | PXD004725 | Pride
Tmt-silac Protein degrdation Tmt Proteome dynamics Silac Human primary fibroblasts Protein synthesis Hyperplexing 
ProteomicsQuantitative proteomics analysis of cumulus cells from the young and old mice
Cumulus cells from the young and old mice were subjected to the TMT6 labeling, HP-RP fractionation, and LC-MS/MS analysis. Cumulus cells surrounding 375 oocytes were collected in each of the three biological replicates. Raw files were analyzed using MaxQuant software.
ORGANISM(S): Mus musculus (Mouse) 
2025-08-25 | PXD050687 | Pride
Mouse Lc-ms/ms Tmt Cumulus cells 
ProteomicsC. elegans LC-MS/MS analysis with TMT labeling
Quantitative spatiotemporal proteomics mapping by Tandem Mass Tag labeling in live C. elegans
ORGANISM(S): Caenorhabditis elegans 
2024-11-05 | PXD027730 | Pride
C. elegans Tmt Lc-ms/ms 
ProteomicsHuman substantia nigra proteome dataset
Quantitative proteomic analysis of human post-mortem substantia nigra from patients with Parkinson’s disease (n=3) versus controls (n=3) based on sixplex TMT (TMT6) isobaric labeling which allowed protein simultaneous identification and quantification. Samples were digested and pooled after TMT6 lab...
ORGANISM(S): Homo sapiens (Human) 
2014-01-28 | PXD000427 | Pride
Human Post-mortem tissue Parkinson's disease Offgel lc/ms/ms orbitrap Hpp brain Substantia nigra 
ProteomicsDirected evolution of TurboID for efficient proximity labeling in living cells and organisms
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY. Nature Biotech 2018. Protein interaction networks and protein compartmentation underlie every signaling process and regulatory mechanism in cells. Recently, proximity labeling (PL) has emerged as a new ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-04-23 | MSV000082304 | MassIVE
ProteomicsIdentification of CTPS1 Filament Interacting Proteins_2019 dataset
CTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Previously, we demonstrated that histidine (His) mediated methylation pro...
ORGANISM(S): Homo sapiens (Human) 
2020-03-24 | PXD015507 | Pride
Apex2 Tmt6 labeling Ctps1 Lc-ms/ms 
ProteomicsA systematic screening pipeline identifies a new regulator of tyrosinated microtubule dynamics
Tubulin detyrosination is a reversible post-translational modification thought to be important for processes including the generation of cell polarity and cell division. The Y/ΔY cycle does not affect the intrinsic properties of microtubules per se, but rather influences the cohort of microtubule-as...
ORGANISM(S): Homo sapiens (Human) 
2022-08-16 | PXD030448 | Pride
Microtubule Detyrosination Tmt6 Human Tyrosination Lc-ms/ms 
ProteomicsPhosphoproteome profiling using the isobaric carrier without the need for phosphoenrichment
Phosphorylation is a crucial component of signaling cascades in a cell. It controls various biological cellular functions such as cell growth and apoptosis. Due to the low stoichiometry of phosphorylaion, enrichment of phosphopeptide prior to LC-MS/MS is necessary for comprehensive phosphoproteome a...
ORGANISM(S): Homo sapiens (Human) 
2022-03-07 | PXD028490 | Pride
Isobaric labeling Tmt Carrier Phosphoproteome Quantitative proteomics 
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