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ProteomicsTAG-TMTpro, a hyperplexing quantitative approach for high-throughput proteomic studies
Isobaric labeling is the most widely used multiplexing quantitative approach in proteomic studies, enabling the comparison of up to 18 samples in a single MS analysis. Expanding the multiplexing capacity is of great necessity for high-throughput proteomic studies. Herein, we establish a novel TAG-TM...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2022-10-14 | PXD033711 | Pride
Proteomics Multiplexing quantitative approach Tmtpro labeling 
ProteomicsTMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing
The development of the TMTpro-16plex series expanded the breadth of commercial isobaric tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described 16plex reagents, the proline-based TMTpro molecule can accommodate two additional combinations of heavy carbon and nitrogen iso...
ORGANISM(S): Homo sapiens (Human) 
2021-04-28 | PXD024275 | Pride
Real-time search Eclipse Byl-719 Pten Kin-193 18plex Tmtpro Mcf10a 
ProteomicsTMTpro: Design and initial evaluation of a novel Proline-based isobaric 16-plex Tandem Mass Tag reagent set
The design and synthesis of a novel proline-reporter-based isobaric Tandem Mass Tag 16 tag set (TMTpro) was carried out and the data uploaded here is a comparison of the performance of the new TMTpro tags with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. Da...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Rattus norvegicus (Rat) 
2019-11-25 | PXD014750 | Pride
Tmt Tmtpro 
ProteomicsOvercoming the detrimental O-acylation in TMTpro labeling improves the proteome depth and quantification precision.
The advent of TMTpro reagents expands the sample multiplexing capacity and enables quantification of up to 18 samples in a multiplexed manner. Similar to classic TMT reagents, TMTpro reagents contain a tertiary amine group in their mole-cules, which markedly enhances their reactivity toward hydroxyl...
ORGANISM(S): Homo Sapiens Saccharomyces Cerevisiae 
2023-12-19 | PXD043778 |
ProteomicsTMTpro16plex Isobaric Labeling Reagents: Attaining New Sample Multiplexing Heights and New Cellular Proteome Depths in Quantitative Proteomics
Isobaric labeling empowers proteome-wide expression measurements simultaneously across multiple samples. Here, an expanded set of 16 isobaric reagents based on a proline backbone (TMTpro) is presented. These reagents have similar characteristics to existing TMT reagents but with increased fragmentat...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD016940 | Pride
Human Tmtpro Proteomics Sps-ms3 Cell lines 
UnknownSynergistic Arg-C Ultra and Lys-C Digestion for Quantitative Proteomics.
Not available
S-EPMC12338676 | biostudies-literature
ProteomicsTMTpro16plex Isobaric Labeling Reagents: Attaining New Sample Multiplexing Heights and New Cellular Proteome Depths in Quantitative Proteomics
Isobaric labeling empowers proteome-wide expression measurements simultaneously across multiple samples. Here, an expanded set of 16 isobaric reagents based on a proline backbone (TMTpro) is presented. These reagents have similar characteristics to existing TMT reagents but with increased fragmentat...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD014369 | Pride
Tmtpro16plex Real-time search Quantitative proteomics 
UnknownLabeled quantitative proteomics dataset of optogenetics induced axon regeneration in mice.
Not available
S-EPMC9167879 | biostudies-literature
ProteomicsNHS-Ester Tandem Labeling in One Pot for 48-Plex Proteomics
Stable-isotope labeling strategies are extensively used for multiplex quantitative proteomics. Hybrid isotope labeling strate-gies that combine the use of isotopic mass difference labeling and isobaric tags can greatly increase sample multiplexity. In this work, we present a novel hybrid isotope lab...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024780 | Pride
48-plex proteomics Tmtpro Mtraq 
ProteomicsIdentification of differentially abundant proteins in glaucomatous and non-glaucomatous trabecular meshwork cell cultures using isobaric multiplexed quantitative proteomics
Glaucoma trabecular meshwork (GTM) cells cultured in vitro retain many characteristics of their in situ phenotype. We used isobaric labeling quantitative proteomics to identify proteins that were differentially regulated between glaucomatous and age-matched non-glaucomatous TM (NTM) cells. NTM (n=5)...
ORGANISM(S): Homo sapiens (Human) 
2026-04-07 | PXD066598 | Pride
Isobaric labeling Trabecular meshwork Glaucoma Mass spectrometry Human Cell culture Quantitative proteomics Extracellular matrix 
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